斑馬魚syndecan-4基因在胚胎發育時期功能之鑑定

Syndecans是一種細胞穿膜蛋白多醣，藉由與細胞間基質或生長因子結合來調節下游的訊息路徑，並影響眾多的生理功能。過去的研究發現 syndecan-4 可以透過醣胺聚多醣鏈 (GAG) 與fibronectin產生交互作用，並在 integrin 的協同作用下，促進 focal adhesion 的生成。除此之外in vitro的實驗中也發現，syndecan-4藉由ectodomain的 NXIP motif 可以影響細胞的延展與移動。儘管對於syndecan-4在哺乳類細胞內所參與的訊息路徑已經有相當程度上的了解，然而對於 syndecan-4 在生物體發育的過程當中所扮演的角色，目前仍不清楚。而近期對於 syndecan-4 的研究發現，似乎開始闡明這個分子對於生物的發育所具有的重要性。研究中指出syndecan-4在胚胎早期發育，似乎扮演著調節神經誘導，及神經脊細胞移動方向的相關機制。因此本篇研究主要希望透過斑馬魚的系統了解發育的過程當中，syndecan-4 對於胚胎發育造成的影響以及其重要性。在研究中發現，過度表現 syndecan-4 的情形下對於斑馬魚的外觀上似乎並不會產生明顯的缺陷；然而當過量表達功能性區域變異的 syndecan-4 時，則發現胚胎發育早期會有肌肉發育異常的現象、脊索產生扭曲以及心血管系統產生變異的情形。針對神經系統的部分研究時，也發現不論是過量表現正常或是功能性變異的 syndecan-4，都可以觀察到在側線系統上的 neuromasts 細胞產生缺失的現象，而這樣的結果證明 syndecan-4 對於斑馬魚的胚胎發育來說，的確對不同種類的細胞組織扮演著重要的角色。

關鍵字

斑馬魚；胚胎發育

並列摘要

Syndecans are a family of transmembrane proteoglycans that are known to modulate a large number of signaling pathway through the interaction with ECM (extracellular matrix) or growth factor and to regulate a great diversity of biological function. Glycosaminoglycan chains of syndecan-4 is responsible for focal adhesion formation in response to ECM coordinated integrin. Results of in vitro experiments suggest that syndecan-4 also modulate cell spreading and migration through NXIP motif of ectodomain. Syndecan-4 signaling and its role in cell adhesion is understood in mammalian cell culture systems. However, little is understood in terms of its role in development. Recently research suggested that syndecan-4 seems to play an important role in organism development. Evidence reveals syndecan-4 is involved in the signaling regulate neural induction and neural crest cells migration during early embryo development. In this study, we aim to unravel the role of syndecan-4 in zebrafish embryo development. Overexpression of syndecan-4 seems to have little defect in morphology, however, overexpression of functional motif-deleted form of syndecan-4 resulted in abnormal muscle distribution and variation of cardiovascular development. The embryo overexpressed normal or mutant form of syndecan-4 also shows the defect of lateral line system in neuraomasts. Taken together, these results suggest that syndecan-4 plays an important role in several types of cells during zebrafish development.

並列關鍵字

zebrafish ； syndecam-4 ； neuromast

參考文獻

Alexopoulou, A. N., Multhaupt, H. A. and Couchman, J. R. (2007). Syndecans in wound healing, inflammation and vascular biology. Int J Biochem Cell Biol 39, 505-28.

Andermann, P., Ungos, J. and Raible, D. W. (2002). Neurogenin1 defines zebrafish cranial sensory ganglia precursors. Dev Biol 251, 45-58.

Chen, E., Hermanson, S. and Ekker, S. C. (2004). Syndecan-2 is essential for angiogenic sprouting during zebrafish development. Blood 103, 1710-9.

Choi, S., Lee, E., Kwon, S., Park, H., Yi, J. Y., Kim, S., Han, I. O., Yun, Y. and Oh, E. S. (2005). Transmembrane domain-induced oligomerization is crucial for the functions of syndecan-2 and syndecan-4. J Biol Chem 280, 42573-9.

Cornelison, D. D., Wilcox-Adelman, S. A., Goetinck, P. F., Rauvala, H., Rapraeger, A. C. and Olwin, B. B. (2004). Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration. Genes Dev 18, 2231-6.

國際替代計量

斑馬魚syndecan-4基因在胚胎發育時期功能之鑑定

全文下載

主題瀏覽