- 學位論文
以奈米螢光粒子分子影像偵測前驅細胞之軟骨化及硬骨化
Novel Detection for Chondrogenesis and Osteogenesis of Progenitor Cells Using Fluorescent Nano-particle Imaging
摘要
軟骨是一種極難自我修復的組織，研究指出可能與軟骨周圍無血管分佈有關，而受傷過後的關節更會有持續退化的趨勢，目前臨床上對於軟骨修復的療法包含替換骨膜、軟骨膜，自、異體移植軟骨組織及鑽孔的方式，但卻都有著很大的限制性。硬骨的再生能力雖然比軟骨好，但如果是大部骨的缺損，或是受傷部位血液灌流不足，自我修復的效果就不明顯，因此臨床上的需求使得研究轉向了間葉幹細胞的移植。 而為了評估及觀測前驅細胞的軟骨與硬骨分化行為，本實驗室發展出兩種探針：螢光奈米粒子與膠原蛋白衍生物IBP分子結合探針(IBP-QD)可偵測軟骨分化；螢光奈米粒子與雙磷酸鹽分子結合探針(BpN-nanoAu)偵測硬骨分化。本篇研究結果顯示，合成探針並純化測試後，利用近內紅外線光學影像顯微系統結合IBP-QD探針與BpN-NanoAu探針，可各別成功地偵測前驅細胞之軟骨分化及硬骨分化。
並列摘要
The repairing efficacy for cartilage and large bone defect are still varied in clinical use. The clinic need for improved treatment has led to investigate in vivo implantation of isolated mesenchymal stem cells (MSCs). Current methods for observing differentiation of MSCs either limited in resolution, efficiency, short life, or health hazard. To define the chondrogenesis and osteogenesis at molecular level, we develop two probes, IBP-QD and BpN-nanoAu, coupling luminescent nano-particles to specific molecule. In chondrogenesis from MSCs, collagen in extracellular matrix is able to media differentiation by specifically binding to integrinα2β1. On the other hand, in matured osteogenesis from osteoblast, bisphosphonates can naturally bind to hydroxyapatite (HA), component of mineralization. Taking together, IBP-QD and BpN-nanoAu probes, which enable to bioconjugate to integrinα2β1 and HA respectively, provide a novel technology to detect the chondrogenesis and osteogenesis at molecular level.