飲食中不同油脂合併補充枸杞多醣萃取物對四氯化碳誘發肝纖維化大鼠之影響

Effects of different dietary oils combined with Lycium barbarum polysaccharide extract on liver fibrosis induced by CCl4 in rats

江昀芸(Yun-Yun Chiang)

指導教授 ： 趙振瑞

臺北醫學大學/營養學院/保健營養學研究所/碩士(2017年)

摘要

目前肝纖維化還未有有效的治療方式，而先前研究指出，橄欖油與枸杞多醣體皆具有保護肝臟的作用，本次實驗欲探討飲食中不同油脂合併枸杞多醣萃取物(Lycium barbarum polysaccharide extract, LBE)補充，對於四氯化碳(carbon tetrachloride, CCl4)誘發肝纖維化大鼠之發炎反應、細胞凋亡及纖維化的影響。雄性Sprague-Dawley 大鼠隨機分成七組：正常飲食組(4%大豆油飲食)、大豆油飲食組(4%大豆油飲食+ CCl4)、橄欖油飲食組(4%橄欖油飲食+ CCl4)、大豆油/橄欖油飲食組(2%大豆油/2%橄欖油飲食+ CCl4)、大豆油飲食+ LBE組(4%大豆油+ LBE + CCl4)、橄欖油飲食+LBE組(4%橄欖油+ LBE + CCl4)、大豆油/橄欖油飲食+ LBE組(2%大豆油/2%橄欖油飲食+ LBE + CCl4)。LBE給予量為每天50 mg/kg bw，預先給予油脂或/和LBE一週後，每週腹腔注射1-2 mL/kg bw誘發肝纖維化，共注射八週。結果顯示注射四氯化碳之六組有較嚴重的肝臟脂質堆積、細胞死亡、發炎反應及纖維增生，含有橄欖油的飲食可降低肝臟TGF-β1與TIMP-1濃度；給予LBE可降低肝臟caspase-9與caspase-3活性，降低肝臟TNF-α濃度，增加IL-10/TNF-α比值，降低肝臟TGF-β1與TIMP-1濃度；當橄欖油與LBE合併給予，可降低肝臟caspase-9與caspase-3活性、降低肝臟TNF-α濃度，增加肝臟IL-10濃度與IL-10/TNF-α比值，降低肝臟TGF-β1與hydroxyproline濃度。對於注射四氯化碳8週之大鼠，枸杞多醣萃取物可改善細胞凋亡、發炎反應及纖維化指標，而橄欖油合併枸杞多醣萃取物的改善效果更佳。

關鍵字

橄欖油 ； 枸杞多醣體 ； 細胞凋亡 ； 發炎反應 ； 肝纖維化

並列摘要

The effective therapy for liver fibrosis is still elusive. Olive oil and Lycium barbarum polysaccharides were considered to act as hepatoprotective activity. This study investigated anti-inflammatory, anti-apoptosis and anti-fibrotic effects of different dietary oils and Lycium barbarum polysaccharide extract (LBE) on CCl4-induced liver fibrosis in rats. Male Sprague-Dawley rats were divided into 7 groups: normal diet (4% soybean oil diet), soybean oil diet (4% soybean oil diet + CCl4), olive oil diet (4% olive oil diet + CCl4), soybean oil/olive oil diet (2% soybean oil / 2% olive oil diet + CCl4), soybean oil diet + LBE (4% soybean oil diet + LBE + CCl4), olive oil diet + LBE (4% olive oil diet + LBE + CCl4), and soybean oil/olive oil diet + LBE (2% soybean oil / 2% olive oil diet + LBE + CCl4). The LBE-treated groups were given 50 mg/kg/day LBE. Rats were injected with 1-2 ml/kg bw/wk CCl4 intraperitoneally for 8 weeks. The results showed that CCl4 caused more severe liver fatty change, cell death, inflammation, and collagen generation. The groups treated with olive oil reduced hepatic TGF-β1 and TIMP-1 concentrations. LBE reduced hepatic caspaspe-3 and caspase-9 activities, decreased the hepatic TNF-α level, increased IL-10/TNF-α ratio, and reduced hepatic TGF-β1 and TIMP-1 concentrations. The groups treated with olive oil combined with LBE reduced hepatic caspaspe-3 and caspase-9 activities, decreased hepatic TNF-α level, increased hepatic IL-10 level and IL-10/TNF-α ratio, as well as reduced hepatic TGF-β1 and hydroxyproline concentrations. Therefore, after CCl4 injection for 8 weeks in rats, LBE improves apoptotic, inflammatory and fibrotic markers, while olive oil combined with LBE has better effects on anti-apoptosis, anti-inflammation and anti-fibrosis.

並列關鍵字

olive oil ； Lycium barbarum polysaccharides ； apoptosis ； inflammation ； liver fibrosis

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