具生物可降解多孔奈米複合材於手術填充物之特性研究

針對創傷或是手術導致之患部出血，一具有高孔洞性之生物可降解性多功能止血棉對於止血以及傷口癒合能有莫大的幫助。本研究的目的為利用止血棉吸取大量的血液，利用血液中所含有之生長因子等促進組織修復，同時提供患部之良好三維支架以利組織再生。計畫將以膠原蛋白/甲基纖維素/幾丁聚醣作為基材，使用冷凍乾燥法來製備一新穎止血棉。膠原蛋白為人體細胞外基質中之主要成分，具有構成與修復組織、保持體內酸鹼平衡、水分平衡與電解質平衡等重要生理機能。甲基纖維素/幾丁聚醣止血棉製備步驟先混和不同濃度的甲基纖維素和幾丁聚醣，再加入3 mg/ml膠原蛋白並加入2.5%戊二醛來進行交聯反應，將其膠狀物質冷凍令水結成冰晶，最後經冷凍乾燥的方法使冰晶昇華，製備出具有三維孔洞結構的止血棉。研究製成的甲基纖維素/幾丁聚醣止血棉孔隙率高達95%以上，具備高度可塑性與良好吸血效果。研究中先探討以不同比例混合膠原蛋白/甲基纖維素/幾丁聚醣所製備止血棉之機械性質、物化性質、降解速率。

關鍵字

止血棉；甲基纖維素；幾丁聚醣；膠原蛋白

並列摘要

The trauma and surgery could result to massive hemorrhage. Therefore, a highly porous biodegradable and multifunctional hemostatic sponge shall benefit hemostasis and wound healing. The purpose of this study is to improve wound healing through growth factors and cytokines within the blood by using hemostatic sponge to absorb this blood. The hemostatic sponge also serves as a scaffold to support regeneration of injury site. The blending of collagen/methyl cellulose/chitosan was used to prepare hemostatic cotton by freeze-drying method. As Collagen is one of the major components in extracellular matrix of human body, they attribute to tissue repairing, acid-base balance, water containing, electrolyte balance and other important physiological functions.The methyl cellulose/chitosan hemostatic sponge is first mixed with different concentrations of methyl cellulose and chitosan, 3 mg/ml collagen was added and evenly mixed. In the later process, 2.5% glutaraldehyde for cross-linking reaction is added to colloidal substances in frozen water to form ice crystals, ice sublimation and finally follow by freeze-drying method. The three-dimensional pore structure of the hemostatic sponge is study, and methyl cellulose/chitosan hemostatic sponge porosity up to 95%, with a high degree of plasticity and good vampire effect is analyze. In the study, the mechanical properties, physicochemical properties, degradation rate and biocompatibility of hemostatic sponges prepared in different proportions of collagen/methyl cellulose/chitosan were examined.