- 學位論文
黃耆增加骨骼肌小管肥大及人體運動損傷後生理恢復之探討
Studies on the Astragalus membranaceus Enhanced Myotube Hypertrophy in Skeletal Muscle and Improved Physiological Recovery after Exercise Injury in Human
摘要
草本植物或食物營養因子介入以促進肌肉肥大或蛋白質合成作用，是增加骨骼肌肉量的重要探討方向。本研究以黃耆萃取物為試驗物，以骨骼肌肉細胞 C2C12 細胞株培育為成熟的肌小管後作為實驗平台，觀察培養液中添加黃耆水萃物粉末後是否可促進肌小管肥大及其可能機制，並以動物試驗評估食用黃耆濃縮製劑之安全性後，探討黃耆濃縮製劑粉末補充對於人體運動損傷後的生理恢復情形。研究結果發現含有黃耆水萃物之培養液可經由 PI3K / Akt / mTOR pathway 促進肌小管肥大。運動訓練之 ICR 小鼠每日每公斤體重補充 0.615 或 3.075 g 黃耆濃縮製劑粉末連續 6 週並不會對小鼠的代謝性生化指標及相關組織產生毒害影響。人體運動損傷後每日補充 2.8 g 黃耆濃縮製劑 (黃耆濃縮製劑中之 astragalosides總含量為1.455 mg / g) 可提升人體血液中 insulin-like growth factor-1 (IGF-1) 濃度，且可增加肌肉組織的血含氧量。根據本研究結果，黃耆萃取物是具有促進肌肉肥大的候選草本食材，未來可能作為促進運動表現的營養增補劑。
並列摘要
Herbal supplements and food factors are important resources to investigate about muscle hypertrophy or protein synthesis. We investigated Astragalus membranaceus (AM) whether those herbal supplements induce hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Then, we study the safety assessment and exercise ability of AM supplements which can promote skeletal muscle hypertrophy. The results revealed that AM can promote hypertrophy in myotubes through the PI3K/Akt/mTOR pathway. Biochemical parameters and histopathological examination revealed no toxic effect of 6-week AM (0.615 and 3.075 g / kg B.W. / day) administration in training ICR mice. Furthermore, AM supplement (2.8 g / day, contain total astragalosides 1.455 mg / g ) can enhance serum insulin-like growth factor-1 (IGF-1) concentrations and increase the oxygen content of muscle tissue in human. According to our study, we suggest that AM may be used as a candidate nutritional supplement to promote athletic performance.