Cycloartenyl ferulate、cycloartenol與ferulic acid交互作用對大腸癌細胞生長之影響

The interaction effects of cycloartenyl ferulate, cycloartenol and ferulic acid on the growth of colon cancer cells

簡均安(Chun-An Chien)

指導教授 ： 施純光

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2012年)

https://doi.org/10.6831/TMU.2012.00215

摘要

國內大腸癌發生率位居癌症發生率首位，增加全穀類攝取能降低大腸癌的風險。米 (Oryza sativa) 在台灣為主食來源之一，米麩中含有豐富的抗癌物質可以預防癌症的發生，如γ-oryzanol、酚類物質、植物固醇。米麩中γ-Oryzanol是由steryl ferulates所構成的混合物，其中含量最多的為cycloartenyl ferulate (CAF)，CAF經由大腸細菌酵素分解為cycloartenol (CA) 及ferulic acid (FA)。本研究欲探討 CAF、CA及FA交互作用對大腸癌細胞HT-29生長抑制的作用。結果顯示CAF、CA及FA可降低細胞存活率，於 「500 μM FA與80 μM CAF」、「500 μM FA與20 μM CA的組合」及「500 μM FA、80 μM CAF及20 μM CA三者合併給予」有較佳的協同作用 (synergistic effect)，增加sub G1細胞數，增加凋亡細胞比例，活化caspase 3，但未調控細胞週期進展。以上結果證實CAF與FA合併給予、CA與FA合併給予、三者合併給予可抑制HT-29細胞生長、促進細胞凋亡，達到抑制大腸癌的作用。

關鍵字

cycloartenyl ferulate、cycloartenol、ferulic acid、交互作用、凋亡、大腸癌細胞

並列摘要

Colon cancer is a leading cause of morbidity in adenocarcinoma in Taiwan. Higher frequency of whole grain food intake has been suggested as an indicator of reduced risk of colon cancer. Rice (Oryza sativa) is a staple food in Taiwan. Rice bran has been shown to contain various anti-cancer substances for preventing cancer, such as γ-oryzanol, phenolic compounds and phytosterols. γ-Oryzanol is a steryl ferulate mixture from rice bran. Cycloartenyl ferulate (CAF) is a major compounds of γ -oryzanol. CAF is metabolized to cycloartenol (CA) and ferulic acid (FA) by microbial enzymes in colon. This study investigated the interaction effect of CAF, CA and FA on the growth inhibition of colon cancer cells. The results showed that CAF, CA and FA reduced the cell viability. Co-treatment of 80 μM CAF and 500 μM FA as well as 20 μM CA and 500 μM FA had better synergistic effects in increasing cells in sub G1 phase and apoptotic cells. In addition, these co-treatment activated caspase-3 but did not modulate progression of cell cycle. These results suggest that CAF-FA co-treatment, CA-FA co-treatment and CAF-CA-FA co-treatment may inhibit cell growth and induce cell apoptosis in HT-29 cells to inhibit colon cancer.

並列關鍵字

cycloartenyl ferulate, cycloartenol, ferulic acid, interaction, apoptosis, colon cancer cells

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