- 學位論文
紫蘇油對卵巢切除大鼠其骨質流失之影響
Effects of Perilla Oil on Bone Loss in Ovariectomized Rats
摘要
高齡及婦女均為骨質疏鬆症重要的危險因子，停經後婦女雌激素的缺乏會導致骨質流失與肥胖的發生。骨重塑對維持骨質密度、骨的大小、形狀以及完整性十分重要。研究顯示膳食油脂中n-3/n-6 脂肪酸比偏低會導致骨質疏鬆症的發生及增加組織的發炎反應，而增加飲食中n-3 脂肪酸含量可以抑制破骨細胞的生成及骨質的流失。臺灣約有10%的素食人口，而膳食n-3 脂肪酸的補充主要來源為魚油，無法建議素食者補充，紫蘇油也富含n-3 多元不飽和脂肪酸，因此可做為 素食者n-3 脂肪酸的良好來源。卵巢切除(ovariecromized; OVX)大鼠為經常被使用的一種因雌激素缺乏而導致骨質疏鬆症的實驗模式，本研究的主要目的是探討紫蘇油對於卵巢切除大鼠其骨質密度的影響。實驗共使用50 隻雌性Sprague Dawley 大鼠，分成五組，包括正常控制組(NC)、卵巢切除對照組(OC), 卵巢切除魚油組(OF), 卵巢切除紫蘇油組(OP2; n-3/n-6 脂肪酸比為1/2) 以及卵巢切除紫蘇油組(OP4; n-3/n-6 脂肪酸比為1/4)，分別餵養十六週後收集動物的血液、肝臟、股骨進行分析。結果顯示，OP2 組的體重低於其他OVX 組，血漿中GOT及GPT 活性在OC 及OF 組較OP2 及OP4 組高，其結果與肝臟病理組織切片相似，顯示紫蘇油可減輕OVX 大鼠肝臟的損傷﹔與NC 組比較，OVX 鼠有較低的雌激素濃度，且其骨質百分比及骨小樑數目較低、骨小樑分離度較高，血漿中骨鈣素(osteocalcin; OCN)濃度在OVX 組顯著高於NC 組，血漿中OCN 濃度增加與骨密度增加呈現正相關，然而在本實驗中紫蘇油組的OCN 濃度顯著低於OC 組 而骨小樑寬度顯著高於OC 組，顯示紫蘇油可能可調節骨的重塑以減少骨質流失。與玉米油及魚油組比較，膳食紫蘇油的補充可減少肝臟的損傷及調節骨質的形成，然而對於骨質流失的影響效果有限。
並列摘要
The most important risk factors for osteoporosis are advanced age and female sex. Estrogen deficiency contributes bone loss and adiposity in postmenopausal women. Bone remodeling is crucial for maintenance of bone mass density (BMD), size, shape and integrity. The source of a lower n-3/n-6 fatty acids ratio in dietary oil is implicated in causing osteoporosis and tissue inflammation. Several studies have shown that increasing diet content of n-3 fatty acids will inhibit osteoclast production and reduce bone loss. The vegetarian population in Taiwan is about 10%. Fish oil is rich in n-3 polyunsaturated fatty acids, but cannot be recommended for vegetarian. The perilla oil is also rich in n-3 fatty acids; it can be a good plant n-3 source for vegetarian. Ovariecromized (OVX) rat was used to be an estrogen-deficiency osteoporotic model. Thus, this study was aimed at studying the effects of perilla oil on inflammatory response and bone mass density in OVX rats. Fifty female Sprague Dawley rats were divided in to five groups: normal control (NC), OVX control (OC), OVX fish oil (OF), OVX -perilla oil with n-3/n-6 ratio 1/2(OP2) and OVX perilla oil with n-3/n-6 ratio 1/4(OP4) groups. After 16 weeks of treatment, the animals were scarified and blood, liver and femur were collected for analysis. The results showed that the OP2 group had a lower body weight than the other OVX groups. The level of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in plasma were higher in the OC group than in the OP2 and OP4 groups. It is consistent with the result of pathology of liver with H&E stain; perilla oil can alleviate the hepatic injury in OVX rats. Compare with the NC group, OVX rats had a lower estradiol level in plasma as well as percent of bone volume, trabecular number and higher trabecular separation. The plasma osteocalcin (OCN) concentration was higher in the OVX rats than NC group for bone formation. It has been observed that higher plasma OCN levels are relatively well correlated with increases in bone mineral density. However, perilla oil groups had a lower OCN level and a higher trabecular width than those in the OC group, maybe can regulate the bone remodeling to reduce bone loss. These results indicated that compare with OVX fed with corn oil and fish oil, dietary perilla oil administration may attenuate hepatic injury and regulate bone formation. But the bone loss is not obviously affected by the perilla oil supplementation in this study.
參考文獻
延伸閱讀
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