二氫比啶誘發之牙齦纖維母細胞過度增生的機轉中IL-6/STAT3訊息傳遞鏈之自動交互作用

IL-6/STAT3 Autocrine Signaling Pathways in The Cells Derived from Dihydropyridine Induced Gingival Overgrowth

粘正宗(Cheng-tzung Nien)

指導教授 ： 呂炫堃

臺北醫學大學/口腔醫學院/牙醫學系碩博士班/碩士(2009年)

https://doi.org/10.6831/TMU.2009.00149

摘要

團隊近年的研究發現IL-1β為二氫比啶（dihydropyridine）促進牙齦過度增生(dihydropyridine induced gingival overgrowth,　DIGO)的主要的促發炎細胞激素。在DIGO細胞中，IL-1β刺激後會增加IL-6和睪固酮受器(androgen receptor,AR)之mRNA表現。而分別抑制IL-6或抑制AR皆會降低總膠原蛋白(total collagen)的生成量，並降低結締組織生長因子(Connective tissue growth factor,CTGF)或第一型膠原蛋白(type I collagen)的mRNA表現。故IL-6與AR是影響牙齦增生機轉中的重要因素。本研究欲探討牙齦增生細胞之IL-6/gp130/STAT3訊息傳遞鍊與AR之關聯。實驗方法將健康的牙齦纖維母細胞與DIGO細胞，培養在charcoal stripped serum中，分別以不同濃度與不同刺激時間的IL-6刺激，進行西方墨點法與雷射共軛焦顯微鏡檢測STAT3的磷酸化與AR的蛋白質表現與位置。結果顯示健康的牙齦纖維母細胞與DIGO細胞STAT3磷酸化皆於15分鐘內達到高峰，且DIGO細胞受IL-6刺激產生的STAT3磷酸化作用較強。AR受IL-6刺激後的反應在本刺激條件下未產生顯著差異。需進行更進一步的實驗探討STAT3與AR之間的交互作用。

關鍵字

二氫比啶 ； 牙齦纖維母細胞 ； 過度增生 ； IL-6 ； STAT3

並列摘要

Our previous studies found that IL-1βis the main cytokine which induces Dihydropyridine Induced Gingival Overgrowth (DIGO) in patient with hypertension. Besides, there were more mRNA expressions of IL-6 and androgen receptor(AR) after stimulation of IL-1β in DIGO cells than healthy cells. Therefore, we supposed that IL-6 and AR are main factors of dihydropyridine induced gingival overgrowth. The propose of this study is to find the relationship of IL-6/STAT3 signaling pathway and AR in DIGO cells. Two types of gingival fibroblast, DIGO cells and Healthy cells, were cultured in charcoal stripped serum and treated with different time and dose of IL-6 cytokine. The result of Western blot and Laser confocal microscopy shows that IL-6 induced STAT3 phosphorylation was occurred in 15 minutes and the phosphorylation peak is between 3 and 15 minutes. The STAT3 phosphorylation is more significant in DIGO cells than healthy cells. However, the change of AR is not significant in this study. We have design further experiments to analysis the relationship between AR and IL-6/STAT3 signaling pathway.

並列關鍵字

IL-6 ； STAT3 ； Dihydropyridine Induced Gingival Overgrowth

參考文獻

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