工業廢水中含有許多酚化合物,如不加以處理而直接排放,會對整個生態環境造成嚴重的污染,所以本研究以中空纖維膜生物反應器中以Pseudomonas putida降解中、高濃度之酚溶液。此系統之優勢在於:對於毒化性、抑制性物質的忍受能力也較傳統式或懸浮式生物處理法高,以期許能夠將酚處理到合乎於排放標準。 建立P. putida於中空纖維膜生物反應器之降解實驗,探討不同組成分、pH值、分散劑、初始細胞濃度、不同預培養方式與流速等對降解速率之影響,找出其實驗最適化的操作條件。由實驗發現,將實驗操作在兩反應器都添加培養機質及分散劑且將管側及殼側流速分別控制在2.4 mL/min,2 mL/min、pH 7.1、OD = 0.06左右和在預培養以100 mg/L下活化其效果最佳。然後將上述之條件運用在不同基質降解實驗下,發現廢水中可降解酚濃度範圍(100 ~ 2800 mg/L)與降解時間(11 ~ 98 h)。分散劑在本實驗扮演著兩種角色,其一為改善細胞阻塞情況,其二為可減低毒化之現象。
Phenolic compounds were often encountered in industrial effluents. It is a serious pollution problem while discharging such wastewater without treatment. The degradation of medium-to-high-strength phenol by pseudomonas putida in hollow fiber membrane bioreactor was thus investigated. The ability of this system to tolerate the toxic and restrained material is better than that of the traditional and freely suspended treatments. Hopefully, this system could meet the standard of effluent discharge. In hollow fiber membrane bioreactors, the degradation rates of phenol were determined as a function of wastewater composition, pH value, with and without dispersion agent, initial cell concentration, with and without pre-culture, and flow rate. The experimental results showed that the degradation performance was the best when the mineral medium and dispersion agent were added in two phases at pH 7.1 and a flow rate of 2.4 (tube side) and 2.0 mL/min (shell side). The performance was enhanced when the cell is pre-cultured in a medium containing 100 mg/L of phenol. Under the above-mentioned conditions the experiment showed that the degradation time was 11 — 98 h in substrate concentration of in the range of 100 — 2800 mg/L. However, the dispersion agent could improve the situation of the cells retards and lower the toxicity.