重金屬鎘污染土壤環境中微生物之分離與鑑定

Identification and Characterization of Cadmium Resistant Microorganisms from Contaminated Soil Identification and Characterization of Cadmium Resistant Microorganisms from Contaminated Soil Identification and Characterization of Cadmium Resistant Microo

洪鈞煒(Chun-Wei Hung)

指導教授 ： 簡志青

元智大學/工程學院/生物科技暨生物資訊研究所/碩士(2005年)

摘要

無資料

關鍵字

重金屬污染 ； 微生物多樣性 ； 原子吸收光譜儀

並列摘要

Abstract

Identification and Characterization of Cadmium Resistant Microorganisms from Contaminated Soil

Due to the overexploitation and industrialization, heavy metal contaminations become one of the major causes of environmental hazard. Therefore, decontamination of heavy metal in the soil and water become more and more important. In this study, we used culture-dependent and culture-independent method to obtain information about microbial diversity in soil which is polluted by heavy metals. Among the bacteria isolated, we cultivated a bacterium that can grow on high concentration of heavy metal. We further study is ability to resist heavy metals as well as its ability to absorb the heavy metals. An isolate from this research, strain CD02 (Stenotrophomonas sp.) has the ability to grow on medium containing cadmium up to 3500μM. According to the results of Atomic Absorption Spectrometry, this bacterium can absorb up to 80 % of cadmium (when grown on medium containing Cd≦3000μM) in the solution. When grown on medium containing Cd, CD02 produced several new proteins compared to those grown on medium without heavy metals. Further experiments will be focus on identification of these proteins and elucidate their role in heavy metal resistance.

Key Word : Heavy Metal Pollution、Microbial Diversity、Atomic Absorption Spectrometry.

並列關鍵字

Heavy Metal Pollution ； Microbial Diversity ； Atomic Absorption

參考文獻

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