聚羥基烷酸生產菌株的快速檢測 、分離與鑑定

A rapid screening, isolation and identification procedure for polyhydroxyalkanoates-producing bacteria

莊育泉(Yu-Chuan Chuang)

指導教授 ： 康世旭

元智大學/工程學院/生物科技暨生物資訊研究所/碩士(2005年)

摘要

微生物在營養不均衡環境下，將多餘的碳源以polyhydroxyalkanoates（PHAs）形式儲存於菌體。PHAs其物理性質與石化塑膠相近，可取代現有泛用塑化原料，並具有很好的生物可分解性與生物相容性。由於PHAs生產成本比石化塑膠高出許多而限制其應用及發展潛力，開發高產率且能夠產生不同性質PHA的菌株，可提升生物可分解塑膠的實用性與普及性。本研究主要目標在於發展一套快速篩選程序，自台灣環境中搜尋高PHAs產率菌株，進一步利用FTIR、GC及NMR等工具分析菌株所生產的PHAs，以搜尋能夠生產不同性質PHAs的菌株。目前已找到29株高PHA產率的菌株，且所合成PHA屬有較高工業應用價值的PHB-co-HHx，本論文確實提供了一快速且有效的篩程序。

關鍵字

聚羥基烷酸 ； 生物相容性 ； 生物可分解性

並列摘要

Polyhydroxyalkanoates (PHAs) is synthesized and accumulated as carbon/energy storage material in microorganisms under the condition of limiting nutrients in the abundance of carbon source. In addition to the good biodegradability and biocompatibility, the physical property of PHAs is similar to synthetic polymers of petroleum origin. Thus PHAs can be used as a substitute for conventional plastic material. However, the cost of PHAs production is much more expensive than that for petrified plastic, posing a restriction to its application and potential development. Establishing a PHA-synthesizing bacterial strain with high productivity and ability to produce heterogeneous forms of PHAs is a feasible strategy to promote the utility and popularity of such biodegradable plastics. The objective of this thesis was mainly to develop a set of rapid screening procedure for bacterial strains producing a high yield of PHAs from the environment in Taiwan. Synthesized PHAs were then subjected to FTIR, GC, and NMR analyses to identify specific bacterial strain(s) capable of yielding heterogeneous forms of PHAs. The result included that twenty-nine bacterial strains with high productivity of PHA synthesis were isolated, which PHA products were biodegradable copolymer P(HB-co-HHx), highly valuable in industrial application. Therefore, the rapid procedure provided in this thesis is demonstrated to be effective for the screening of PHA-synthesizing bacteria.

並列關鍵字

PHAs ； biodegradability ； biocompatibility

參考文獻

黃晉貴（2005）台灣本土根瘤菌Wautersia taiwanensis 184生產聚羥基丁酸醱酵策略之探討. 元智大學生物科技暨生物資訊研究所碩士論文

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