利用酵素降解幾丁聚醣之動力學研究

Degradation Kinetic of Chitosan using Enzyme

鍾建中(Chung-Chen Chung)

指導教授 ： 吳和生

元智大學/工程學院/化學工程與材料科學學系/碩士(2004年)

摘要

摘要
近年來，葡萄糖胺在治療退化性關節炎這方面相當具有療效，而葡萄糖胺的相關產品漸漸的開始普及，葡萄糖胺的產品療效與藥物性質，很少討論葡萄糖胺的製造流程與方式，故本研究主要是利用生物性的酵素降解幾丁聚醣，利用酵素加速降解反應，討論製備葡萄糖胺的可行性以及降解反應在動力學上的探討。目前是利用呈色法與HPLC(High Performance Liquid Chromatography)的來檢測是否在降解反應後會產生葡萄糖胺，經過這2種方法檢測後，可以確定含有葡萄糖胺，再以測量粘度的方式探討幾丁聚醣在不同種類酵素(bromelain、papain、lipase等)、不同酵素含量、不同轉速以及不同溫度下降解反應之動力學探討。
在葡萄糖胺的檢測這方面利用了2種方法(呈色法與HPLC法)可以確定Papain(form papaya latex)降解反應出來的產物中含有葡萄糖胺，而Bromelain(from pineapple stem)雖然會對幾丁聚醣的黏度迅速的減少，但無法在HPLC的測試中得到應有的圖譜，所以目前無法證明幾丁聚醣在Bromelain(from pineapple stem)的反應下，會產生葡萄糖胺。
經過DEAE(diethylaminoethyl) column分離純化後的Papain(form papaya latex)與Bromelain(from pineapple stem)，可以確定Papain(form papaya latex)與Bromelain(from pineapple stem)並不是單一的蛋白質酵素，經純化分離後所得的結果，這2種蛋白質酵素Papain(form papaya latex)與Bromelain(from pineapple stem)裡面含有2種成分的蛋白質。
關鍵字：幾丁聚醣、葡萄糖胺、木瓜蛋白酵素、鳳梨蛋白酵素、降解反應。

關鍵字

幾丁聚醣 ； 葡萄糖胺 ； 木瓜蛋白酵素 ； 鳳梨蛋白酵素 ； 降解反應

並列摘要

Abstract
Nearly years, glucosamine salts have good curative effect in arthritis. Then glucosamine products have begin available to all. When I compare references that about glucosamine products, most references almost refer to curative effect of product and property of medicine, but discussed in process and how to made glucosamine that is very deficient. So this research that mainly confer to use bio-enzyme degraded chitosan and increase degradation of reaction. We focus on made up glucosamine discuss the research feasibility and discuss kinetics of degradation. Now we used color test and HPLC(High Performance Liquid Chromatography) that 2 methods measure glucosamine in degradation of chitosan. After this 2 kind of determination, we can confirm the glucosamine exist in residual degradation of chitosan, and used to Ubbelohde capillary viscometer, and measured time (ηt ) at 25℃ in circular bath. By measuring time (ηt ), we discussed the kinetics in different conditions(different ).
In this research，we make sure Papain(form papaya latex) that can produce glucosamine in degradation reaction of chitosan by proving to color test and HPLC determination. Bromelain (from pineapple stem) cause to decrease viscosity of chitosan in the reaction, but bromelain (from pineapple stem) have no peak in HPLC determination. Now, we can’t make sure bromelain (from pineapple stem) that can produce glucosamine in degradation reaction of chitosan.After DEAE (diethylaminoethyl) column separating papain(form papaya latex) and bromelain(from pineapple stem), we can make sure that they are not single protein of enzyme. Papain(form papaya latex) and bromelain(from pineapple stem) both have 2 components of the protein.
Key word: chitosan, glucosamine, papain(form papaya latex), bromelain(from pineapple stem), degradation reaction.

並列關鍵字

chitosan ； glucosamine ； papain(form papaya latex) ； bromelain(from pineapple stem) ； degradation reaction

參考文獻

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