溫度敏感型甲基纖維素水膠之成膠行為與間葉幹細胞在其中之生物相容性

Thermosensitive methylcellulose hydrogels：its gelation behavior and biocompatibility with MSCs

張雅婷(Ya-Ting Chang)

指導教授 ： 孫ㄧ明

元智大學/工程學院/生物科技暨生物資訊研究所/碩士(2006年)

https://doi.org/10.6838/YZU.2006.00172

摘要

本研究探討溫度敏感性甲基纖維素水溶液之相轉移情形和成膠動力學，並測試間葉幹細胞在甲基纖維素水膠中之生物相容性。添加0.13 M NaCl至甲基纖維素水溶液可產生鹽析作用，而降低成膠溫度及提升成膠速率。高濃度甲基纖維素溶液，因鏈間糾纏造成相轉移溫度下降及成膠速率提高。甲基纖維素溶液至9 wt%以上，成膠溫度趨近於42℃，而藉由鹽類添加造成成膠溫度降低而趨近至40℃。在細胞培養部分，當間葉幹細胞培養於3D甲基纖維素水膠中，可觀察到細胞聚集現象，並且能夠存活至28天，證實甲基纖維素水膠具生物相容性，為可應用於組織工程之注射型水膠。

關鍵字

甲基纖維素 ； 間葉幹細胞 ； 水膠

並列摘要

The phase transition behaviors and the gelation kinetics of the thermosensitive methylcellulose (MC) solution were studied and the MC gels were tested for the biocompatibility with mesenchymal stem cells (MSCs). The presence of NaCl (0.13 M) decreased the gelation temperature (or cloud point) and increased the rate of the gelation process due to the salting out effect. The gelation temperature decreased and the rate of gelation increased with the increase of MC concentration in the solution due to the chain entanglement effect at higher polymer concentrations. The gelation temperature of the salt-free MC solution approximated to 42 oC and that of the MC solution with 0.13 M NaCl approximated to 40 oC when the polymer concentration was higher than 9 wt%. When MSCs were cultured within the 3D MC gels, it was found that MSCs could aggregate and maintain their viability up to 28 days. The biocompatibility of MC gels with MSCs suggested that MC gels could be considered as an injectable hydrogel for tissue engineering application.

並列關鍵字

methylcellulose ； mesenchymal stem cells (MSCs), ； hydrogel

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