海洋環境中生產聚羥基烷酸微生物之探討

Isolation and identification of microorganisms produce poly-hydroxybutyrate from marine environment

周孟輝(Meng-Hui Chou)

指導教授 ： 簡志青

元智大學/工程學院/生物科技暨生物資訊研究所/碩士(2006年)

https://doi.org/10.6838/YZU.2006.00180

摘要

從海洋環境中分離並篩選出可以累積PHA的微生物。篩選出的四株弧菌屬的菌種(M11、M14、M20、M31)在不同的碳源下(包括acetate, glycerol, succinate, glucose, sucrose)都可以合成胞內的脂質堆積。在氣相層析儀和核磁共振儀的分析中顯示，胞內的脂質堆積只有poly-ß-hydroxybutyrate，在篩選出來的菌種當中沒有其他polyhydroxyalkanoates的累積，這樣的結果顯示polyhydroxyalkanoates的多樣性在海洋環境中可能並沒有比在其他環境中來的高。當M11生長在含有4%鹽的培養基下，其累積的Poly-ß-hydroxybutyrate與乾菌重比可以到達41%。而M11的部分16S rRNA序列比對最接近Vibrio natriegens 和 Vibrio parahaemolyticus。

關鍵字

聚羥基烷酸；弧菌屬

並列摘要

Microorganisms isolated from marine sediments were screened for their ability to accumulate polyhydroxyalkanoates. Four Vibrio spp. (strain M11, M14, M20 and M31) were characterized to be able to synthesize intracellular lipid inclusions using various carbon sources including acetate, glycerol, succinate, glucose and sucrose. Gas chromatography analysis and nuclear magnetic resonance analysis reveals the inclusions were poly-ß-hydroxybutyrate. No other type of polyhydroxyalkanoates was found in these marine isolates, which indicate the diversity of polyhydroxyalkanoates produced in marine environment may not as versatile as other environments. Poly-ß-hydroxybutyrate accumulated in strain M11 can be as high as 41% of cell dry weight when cell grown in medium containing 4% of salts. The partial 16S rRNA sequences suggest these strains are closely related to Vibrio natriegens and Vibrio parahaemolyticus.

並列關鍵字

polyhydroxyalkanoates；Vibrio sp.

參考文獻

A. J. Anderson, E. A. Dawes, 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates, Microbiological Reviews, 54(4) 450-472

B. H. A. Rehm, 2003. Polyester synthases: natural catalysts for plastics, The Biochemical Journal, 376 15-33

D. Y. Kim, T. Luぴtke-Eversloh, 2005. Poly(3-mercaptopropionate): A nonbiodegradable biopolymer?, Biomacromolecules, 6(2) 897-901

F. E. Dewhirst, C. C. Chien, 1999. Phylogeny of the defined murine microbiota: Altered Schaedler Flora, Applied and Environmental Microbiology, 65 (8) 3287-3292

G. DU, J. Y U, 2002. Green technology for conversion of food scraps to biodegradable thermoplastic polyhydroxyalkanoates, Environmental Science & Technology, 36 (24) 5511-5516