- 學位論文
利用工業製程廢水生產聚羥基烷酸之效能評估
Performance Evaluation of Polyhydroxyalkanoates Production via the Industrial Process Wastewater
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摘要
聚羥基烷酸(Polyhydroxyalkanoates, PHAs)為一生物可分解性高分子，藉由化學結構上的不同長度主鏈與側鏈組合，可發展出超過120種不同的型態與類別。PHAs屬於天然聚酯類(polyester)，主要為細菌在生長過程中，因碳源過剩且其他營養源(如氮源、磷源)缺乏時，細菌將會利用多餘的碳源代謝累積而生成。PHAs似塑膠的特性及化石燃料之枯竭，使其具有取代現有石化塑膠製品之發展潛力。但PHAs工業化之發展，目前仍侷限於其生產成本偏高。因此，降低PHAs生產成本，進而落實其商業化製程，是為本研究之主要動機與目的。本研究嘗試利用各種製程廢水，以生產PHAs，藉以降低其生產成本。本研究首先利用recombinant Escherichia coli XL1-Blue評估其代謝各種製程廢水，以合成PHAs之能力。研究發現，在各種可利用為基質之製程廢水中，以製酒過程中所產生之廢水，對菌體生長及合成PHB有明顯的效果。研究證實：在未經發酵策略的誘導下，Escherichia coli XL1-Blue可累積合成PHB含量達8.87%。但研究進一步顯示：Escherichia coli XL1-Blue若輔以兩階段發酵策略後，其PHB content可進而提升達到35%。由此證明，利用工業製程廢水並輔以不同發酵策略，的確可有效提升累積PHAs含量，藉此結果顯示：工業製程廢水，應用於合成生物高分子PHA並降低其生產成本之可行性。
並列摘要
Polyhydroxyalkanoates (PHAs), a biodegradable polymer, by the different chemical structure on the length of the main chain and side chain composition, can be developed over 120 different types and categories. PHAs are natural polyester, mainly for the growth process of bacteria, due to excess carbon and other nutrient sources (such as nitrogen and phosphorus) deficiency, bacteria will accumulate PHAs by exceeding carbon metabolism. PHAs have the properties of plastic and fossil fuel depletion, to replace the existing petrochemical plastic products with the potential for development. However, PHAs’ application on industrialization is still confined to its high production costs. Therefore, reducing PHAs production costs will be an important issue for its commercialization process in the near future. Thus, this study aims to develop a chip manufacturing process for the production of PHA. We use the recombinant Escherichia coli XL1-Blue to assess their use of processing waste water, and the ability for the synthesis of PHAs. Studies show the method can be used as a substrate in a variety of process wastewater. Besides, the generations of brewery processed wastewater from the cell growth and the PHB synthesis have significant results. Study also confirms that fermentation strategy without induction, Escherichia coli XL1-Blue synthetic PHB content can be accumulated to 8.87%. The study further shows: Escherichia coli XL1-Blue, if supplemented by a two-stage fermentation strategy, the PHB content can be further improved to 35%. It is evident that, using industrial process wastewater and with different fermentation strategies can effectively enhance the accumulation of PHAs content. The results show industrial process wastewater, used in synthetic and biological polymers PHA feasibility of reducing their production costs.
參考文獻
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