環境中分離紫色非硫光合菌及其可能應用之探討

Isolation of environmental photosynthetic purple non-sulfur bacteria and their potential applications

劉宛佐(Wan-Tso Liu)

指導教授 ： 簡志青

元智大學/工程學院/生物科技與工程研究所/碩士(2011年)

摘要

紫色非硫光合菌 (purple non-sulfur bacteria) 在不同環境中可實行不同代謝方式，例如: 光合自營、光合異營、化學自營、化學異營。由於這類的細菌有其自有的生理特性，在加上光合菌本身廣泛之應用於環保、水產養殖、農業種植、畜牧、醫藥等行業， 最近幾年已受到許多研究者的重視。紫色非硫光合菌廣泛的生長在自然界中，不需要太多的養分，只需一些有機物質以及有光照之地便能生長。在本研究中，我們從北台灣附近水池、稻田、水溝等地的土壤或沉積，利用厭氧光照方式培養，分離光合菌株，利用PCR技術擴增分離菌株之16s rDNA，經由定序分析結果，篩選所要進行探討知紫色非硫光合菌(Rhodobacter sp.)。並評估這些本土分離之紫色非硫光合菌株在應用方面之研究，包括Coenzyme Q10含量的分析、細菌色素含量及種類如類胡蘿蔔素等。

關鍵字

紫色非硫 ； 光合菌 ； 類胡蘿蔔素 ； Coenzyme Q10

並列摘要

Purple non-sulfur bacteria (PNSB) possess diverse metabolic pathway to survive in different environments, including photoautotrophic, photoheterotrophic, chemoautotrophic as well as chemoheterotrophic metabolisms. PNSB have been widely used in the fields of environmental protection, agricultural production, aquaculture and medical application, etc. The unique physiological characteristics of PNSB have also attracted the attention of researchers to investigate the possible application of these microorganisms. In this study, we have isolated and identified several strains of photosynthetic bacteria from sediments or soil from different environment in northern Taiwan, including pond water, rice field and channel. Enrichment technology was employed for isolation of PNSB with anaerobic incubation under light condition. The isolates were identified by 16S rRNA gene sequence analysis and several strains of Rhodobacter sp. were chosen for further evaluation for their applications such as production of coenzyme Q10 and bacterial pigments.

並列關鍵字

purple non-sulfur bacteria ； Carotenoid ； Coenzyme Q10

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