This study examined the effects of corporate culture, innovation, and profitability by conducting surveys in samples of private enterprises in Taiwan. Out of the 174 questionnaires sent, 171 valid questionnaires were obtained. The analyzing results indicated that 1) companies with different types of culture had significantly different effects on innovation of companies; 2) companies with different types of culture affected profitability of companies significantly. Generally, the results of this study indicated that the corporate culture had significant influences on innovation and profitability of companies.