生物可降解材料PHBV微球之製備與藥物釋放之研究

A study on the preparation of biodegradable PHBV microparticles and its drug release

蔡志煜(Chih-Yu Tsai)

指導教授 ： 朱一民

元智大學/工程學院/生物科技與工程研究所/碩士(2012年)

摘要

在外科手術中傷口感染所引發之骨髓炎（Osteomyelitis）是一個難以治療且嚴重的問題。使用藥物投遞系統長時間供給患部抗生素，可給予治療並降低感染機率的發生。許多生物可分解高分子被使用在藥物投遞系統中，聚羥基烷酯類（Polyhydroxyalkanoates，PHAs）是近期受到矚目的生物可降解高分子，PHAs中以poly（3-hydroxybutyric acid-co-3-hydroxyvaleric acid）（PHBV）最常被廣泛的使用，由於它具有良好的生物相容性、生物可降解性及熱加工性，且化學結構相似目前常用的生物可降解高分子，例如PLA、PLGA，其降解速率較慢，適合用於長時間的藥物投遞系統。
　　在本研究中以雙乳化溶劑蒸發技術製備PHBV微球，vancomycin/gentamicin包覆率不高。而沉澱析出法可明顯增加其包覆率及負載率，PHVB微粒包覆率26.96％；PHVB/chitosan微粒包覆率34.31％；PHVB/PEG微粒包覆率26.2％。以CHI50之包覆效果最佳。而gentamicin對於vancomycin具有沖提作用，使vancomycin釋放率增加178％。藥物釋放曲線以PEG75初期突釋量較低接著緩慢釋放藥物，具有較適合之藥物釋放行為。

關鍵字

藥物釋放 ； vancomycin ； gentamicin ； PHBV

並列摘要

Infection is one of the most serious problems in implantation surgery. The utilization of drug delivery systems in surgical procedures offers an addition therapeutic approach to the treatment of infection and the possibility of minimization of the systemic use of drug, and delivering an effective antimicrobial at a sufficiently high concentration to the area of infection in combination. Many degradable polymers have been used as drug delivery systems in implantation surgery. Biodegradable and biocompatible materials, Poly(3-hydroxybutyrate) (PHB) and its copolymers poly(hydroxybutyrate-cohydroxyvalerate) (PHBV) are the most widely used poly(hydroxyalcanoates) (PHAs). Gentamicin and vancomycin are the most extensively used to treat osteomyelitis.
In this study, PHBV microparticles without hight drug encapsulation efficiency (E.E.) were prepared by an water-in-oil-in-water (w/o/w) emulsion/solvent evaporation technique. Precipitation method preparation of microparticles increase drug encapsulation efficiency to 26.96%. PHBV/chitosan and PHBV/PEG microparticles drug E.E. to 34.31 and 26.2% separately. Vancomycin elution was enhanced by 178% with the addition of gentamicin.

並列關鍵字

drug delivery systems ； vancomycin ； gentamicin ； PHBV

參考文獻

24. 質藥物釋放之測定,國立成功大學化學工程學系,2006.

1. Chihara, S., MB, and Segreti, J., MD, Osteomyelitis, 2010.

2. Chen, G.Q., A microbial polyhydroxyalkanoates (PHA) based bio- and

3. Chinwetkitvanich, S., Randall, C.W. and Panswad, T., Effects of

sludge. Water Sci Technol, 50(8), 135-143, 2004.