枯草桿菌ilvC的選殖並探討其對表面素生產之影響

Cloning of ilvC from Bacillus subtilis and its influence in surfactin production

吳芝妤(Ze-Yu Wu)

指導教授 ： 簡志清

共同指導教授 ： 康世旭(Shih-Shu Kung)

元智大學/工程學院/生物科技與工程研究所/碩士(2008年)

摘要

表面素為枯草桿菌ATCC21332以非核醣體胜肽合成機制所合成的脂胜肽生物界面活性劑，結構為由七個胺基酸所組成的環狀胜肽鏈，以及ㄧ長碳鏈脂肪酸側鏈所組成。ilvC是枯草桿菌合成Valine、Leucine、Isoleucine 胺基酸所需的酵素，而這些胺基酸為表面素胜肽鏈的主要組成。將ilvC以穿梭質體送入枯草桿菌SWK569，觀察表面素的產量。卻發現表面素產量只有原先的十分之ㄧ，且於固態培養基上發現菌體的活性會快速消失，並且死亡。推測是因為大量表現ilvC，始得菌體的生長受到影響，進而影響表面素的產量。

關鍵字

科草桿菌 ； 表面素

並列摘要

Surfactin is a cyclic lipopeptidic biosurfactant produced by Bacillus subtilis ATCC 21332 with nonribosomal peptide synthesis mechanisn.Surfactin is composed of seven amino acids of lipopeptide and a long-chain fatty acid. Bacillus subtilis need ilvC the enzyme to product Valine, Leucine, Isoleucine, and thesis amino acid are the main to structure the peptide chain。Using shuttle vector ransforming ilvC to Bacillus subtilis SWK569，but the deci-product of SWK569 only。Finding the bereft activity of SWK569 would die on plate。Calculation that was because the over expression of ilvC to effect the physiology of SWK569, and effect the productivity of SWK569。

並列關鍵字

Bacillus subtilis ； Surfactin

參考文獻

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