3α羥基類固醇脫氫酶/羰基還原酶中精胺酸167在寡聚合作用過程中結構與功能角色之探討

Studies on the Structural and Functional Roles of Arg167 in the Oligomerization of 3alpha-Hydroxysteriod Dehydrogenase/ Carbonyl Reductase From Comamonas testosteroni

黃姿蓉(Tzu-Jung Huang)

指導教授 ： 黃啟清

高雄醫學大學/醫學院/生物化學研究所/碩士(2007年)

摘要

無資料

關鍵字

3α羥基類固醇脫氫酶/羰基還原酶

並列摘要

3α-Hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) from Comamonas testosteroni, a member of the short-chain dehydrogenase/reductase (SDR) family, catalyzes the oxidation of androsterone with NAD+ to form androstanedione and NADH. Previously studies have showed the residue Tyr155 acts as a general base in the 3α-HSD/CR catalyzed reaction. Structurally, 3α-HSD/CR is a homodimer. A salt-bridge interaction is formed between the Asp249 in helix αCT of each subunit with Arg167 in helix αF of other subunit based on structural analysis. To explore the structural and functional role of Arg167 in the oligomerization of 3α-HSD/CR, we performed site-specific mutagenesis to replace Arg167 to Ala, Gln and Asp, respectively, and also utilized kinetic assay, spectroscopic measurment, gel filtration chromatography, analytical ultracentrifugation, thermal and urea unfolding experiments. In kinetic assay, kcat/Km of R167A, R167Q and R167D were drastically decreased by 1.5×105-fold,1.5×104-fold and 6×105-fold compared with wild-type, respectively. The secondary structures of the wide-type and mutants of R167A, R167Q, and R167D are slightly different based on the results from the CD spectra, while a red shift is observed in the mutants on fluorescence spectra.The results from gel filtration chromatography and sedimentation velocity indicate a concentration-dependent dimerization of wide-type and mutant enzymes. The study from the thermal and urea unfolding indicates the mutation of the residue Arg167 destabilized the protein structure. In conclusion, the salt-bridge interaction between Asp249 and Arg167 of each subunit is important in stabilizing dimeric formation of 3α-HSD/CR.

並列關鍵字

3α-HSD/CR

參考文獻

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