1、毛細管電泳法對強心配醣體藥物digoxin與digitoxin之同時分析研究 2、毛細管電泳法對人體腦脊髓液和血漿中抗生素cephalosporins第四代藥物cefepime定量分析研究

1.Simultaneous Determination of Digoxin and Digitoxin by Micellar Electrokinetic Chromatography 2.Quantitative Determination of Cefepime in Human Cerebral Spinal Fluid and Plasma by Micellar Electrokinetic Chromatography

曾綉惠(Hsiu-Hui Tseng)

指導教授 ： 陳素惠

高雄醫學大學/藥學院/藥學研究所/碩士(2003年)

摘要

本論文的主題是利用毛細管電泳中微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC）分別針對不同的藥物發展簡單且高感度的分析方法。主要的研究包括強心配醣體藥物的製劑分析，以及針對人體腦脊髓液和血漿中抗生素cephalosporins第四代藥物cefepime加以分析。主要的成果摘錄如下：
1、毛細管電泳法對強心配醣體藥物digoxin與digitoxin之同時分析研究
本論文建立一種簡單的微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC）同時對強心配醣體藥物digoxin與digitoxin之同時分析研究。此方法分析條件為：10 mM Tris緩衝溶液（pH=9.0）、90 mM sodium dodecyl sulfate （SDS）為陰離子介面活性劑。影響分離的參數包括：緩衝溶液的濃度、pH值、以及SDS的濃度均加以探討。此分析方法可應用於digoxin市售注射劑與錠劑之含量分析。
2、毛細管電泳法對人體腦脊髓液和血漿中抗生素cephalosporins第四代藥物cefepime定量分析研究
本論文建立一種簡單的微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC）分別對人體腦脊髓液和血漿中抗生素cephalosporins第四代藥物cefepime分析研究。對於腦脊髓液中分析方法條件為：20 mM Tris緩衝溶液（pH=9.0）、200 mM sodium dodecyl sulfate （SDS）為陰離子介面活性劑。對於血漿中分析方法條件為：10 mM Tris緩衝溶液（pH=8.0）、150 mM sodium dodecyl sulfate （SDS）為陰離子介面活性劑。影響分離的參數包括：緩衝溶液的濃度、pH值、以及SDS的濃度均加以探討。這兩種分析條件可應用於腦脊髓液和血漿中cefepime之含量分析。

關鍵字

毛細管電泳法 ； 強心配醣體 ； 第四代頭孢子素藥物

並列摘要

The purpose of this work is directed to the development of simple and sensitivity methods for the different medicines by micellar electrokinetic hromatography. The main study included simultaneous determination of cardiac glycosides and trace analysis of cefepime in human cerebral spinal fluid and plasma by micellar electrokinetic chromatography. The results are summarized as follow：
1. Simultaneous Determination of Digoxin and Digitoxin by Micellar Electrokinetic Chromatography
A simple micellar electrokinetic chromatography is described for simultaneous determination of digoxin and digitoxin. The simultaneous analysis of digoxin and digitoxin was performed in Tris buffer (10 mM; pH 9) with 90 mM sodium dodecyl sulfate and 10% isopropyl alcohol as an anionic surfactant and organic modifier, respectively. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. Partial application of the proposed method to the determination of digoxin in commercial tablets and in injections proved to be feasible.
2. Quantitative Determination of Cefepime in Human Cerebral Spinal Fluid and Plasma by Micellar Electrokinetic Chromatography
A simple micellar electrokinetic chromatography is described for determination of cefepime in human cerebral spinal fluid and plasma. The analysis of cefepime in human cerebral spinal fluid was performed in Tris buffer (20 mM; pH 9) with 200 mM sodium dodecyl sulfate as an anionic surfactant. The analysis of cefepime in in human plasma was performed in Tris buffer (10 mM; pH 8) with 150 mM sodium dodecyl sulfate as an anionic surfactant. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. Application of the method to the determination of cefepime in human cerebral spinal fluid and plasma proved to be feasible.

並列關鍵字

capillary electrophoresis ； digoxin and digitoxin ； cefepime

參考文獻

1. Introduction to Capillary Electrophoresis, Modes of capillary electrophoresis, Beckman, p. 12-31

2. R. Kuhn and S. Hoffstetter-Kuhn, Principle of MEKC. Capillary Electrophoresis： Principles and Practice, Spring-Verlag Press, Berlin, 1993, p. 191-198

3. Gilman, A. G., Rall, T. W., Nies, A. S., Taylor, P. (Eds.), Goodman and Gilman’s The Pharmacological Basis of Therapeutics, Pergamon Press, New York, 1991, p. 827.

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