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- 學位論文
芳香烴受體在樹突細胞分化的角色
The Role of Aryl Hydrocarbon Receptor in Dendritic Cell Development
摘要
前言：芳香烴受體（aryl hydrocarbon receptor; AhR）也稱為戴奧辛受體（dioxin receptor），主要功能為進行解毒作用，近年來對於AhR的研究，著重於利用環境因子戴奧辛誘導AhR的表現，發現其可促進樹突細胞的成熟與改變功能，甚至可能影響免疫系統，但AhR本身對樹突細胞的生理角色卻不清楚。 目的：利用 AhR 之基因剔除小鼠，研究 AhR 對於樹突細胞分化與功 能的角色。 實驗方法：利用骨髓細胞進行樹突細胞的分化與功能分析，並分析脾 臟細胞內樹突細胞比例與族群分布情形。 結果：公鼠與母鼠的結果顯示，在骨髓樹突細胞的成熟、死亡比例、 以及其細胞激素的表現皆無顯著上的差異，而在脾臟細胞中的類漿性 樹突細胞（plasmacytoid dendritic cell）和傳統樹突細胞（conventional dendritic cell）的比例與總數、以及其脾臟細胞的總數也沒有任何顯著差異，由目前實驗結果得知，推論AhR分子的活性並不影響樹突細胞的分化。 結論：AhR對整體免疫反應的影響，還需要疾病動物模式或感染模式 作進一步的確認。
並列摘要
Introduction:Aryl hydrocarbon receptor (AhR), the dioxin receptor,plays an important role in detoxification. Recent study shows that AhR activation by dioxin affect dendritic cell (DC) maturation and function in vitro, and alters immune system in vivo; however, the role of AhR on DC differentiation and function is still unknown. Specific aim:To study the physiologic role of AhR on DC differentiation and function using DC-specific AhR knockout mice. Methods: Bone marrow-derived DCs were used to analyze the role of AhR on their differentiation and function. DC subsets in spleen were further examined for DC homeostasis in DC-specific AhR knockout mice. Result: There is no significant difference between DC-specific AhR KO mice and littermate wild type mice, including DC differentiation, cytokine levels in response to LPS, DC subset percentages and numbers. These results suggest that AhR is dispensable for DC differentiation and function. Conclusion:It still needs disease animal models to elucidate the role of AhR in DC functions.