毛細管電泳法對尿液中甲基安非他命與其代謝物之直接分析研究

Direct analysis of methamphetamine and its metabolites in urine by capillary electrophoresis

鄭慧玲(Hui-Ling Cheng)

指導教授 ： 吳秀梅

高雄醫學大學/藥學院/藥學研究所/碩士(2006年)

摘要

本論文針對藥癮者尿液中甲基安非他命(methamphetamine, M)及其主要代謝物安非他命(amphetamine, A)及對位羥基甲基安非他命(p-hydroxymethamphetamine, H)建立簡單靈敏之毛細管電泳法，以提供此濫用藥物之直接篩檢方法；本研究利用長時間電動取樣方式，有選擇性的注入陽離子待測物，並搭配微胞掃集技術堆積檢品提高感度，再以微胞電泳法進行分離；本法不需檢品前處理，即可直接進行分析，實驗方法為先將含有20% (v/v)乙腈之80 mM磷酸鹽緩衝液注入毛細管中，接著以壓力注入一段導電度更高之磷酸鹽緩衝液(100 mM)，再用電動大量取樣(10 kV, 600 s)，最後以含有100 mM硫酸月桂酯鈉及20% (v/v)乙腈之50 mM磷酸鹽緩衝液掃集待測物並進行分離，分離電壓為-20 kV，偵測波長為200 nm。對所建立之方法進行確效，M之監測濃度範圍為100 – 5000 ng/mL、A及H則為100 – 2000 ng/mL，該等檢量線皆呈現良好的線性關係(r ≥ 0.9974)；評估同日間及異日間多次分析結果，其RSD值小於14.3 %，RE值則小於13.3 %，顯示此方法具有良好的精確度及準確性；M之偵測極限為20 ng/mL、A及H則為15 ng/mL(S/N = 3)，較毛細管區帶電泳法約可提升萬倍感度；本法應用於14位M藥癮者尿液之檢測，皆可測得其濃度，部分檢體亦可測得其代謝物，預期本法可應用於快速篩檢藥癮者之檢體。

關鍵字

毛細管電泳 ； 甲基安非他命 ； 代謝物

並列摘要

Methamphetamine (M), amphetamine (A), and p-hydroxymeth- am¬phetamine (H) were determined in urine by cation-selective exhaustive in¬jection and sweeping micellar electrokinetic chromatography (CSEI- sweep-MEKC). A phosphate buffer (80 mM , pH 3) containing 20% acetoni¬trile was filled into an uncoated fused silica capillary (40 cm, 50 μm I.D.), then high conductivity buffer (100 mM phosphate, pH 3; 1 psi for 2.5 min) was followed. Electrokinetic injection (10 kV, 600 s) was used to load samples and to enhance sensitivity. The stacking step and separa¬tion were performed by using phosphate buffer (50 mM, pH 3) con¬taining 20% acetonitrile and 100 mM sodium dodecyl sulfate at -20 kV and 200 nm. Using CSEI-sweep-MEKC, the analytes could be simul- ta¬neously analyzed and have a detection limit down to ppb level. During method validation, calibration plots were linear (r ≥ 0.9974) over a range of 100 – 5000 ng/mL for M, and 100 – 2000 ng/mL for A and H. The lim¬its of detection were 20 ng/mL for M, and 15 ng/mL for A and H (S/N = 3). Our method was applied for analysis of 14 real urine samples. The re¬sults showed good coincidence with immunoassay and GC-MS. This method was feasible for application to detect trace levels of abused drugs in forensic analysis.

並列關鍵字

capillary electrophoresis ； methamphetamine ； metabolites

參考文獻

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