蝦紅素於體內及體外經由紫外線引起傷害之修復途徑研究

紫外光(Ultraviolet, 200~400 nm)在誘導皮膚疾病，例如皮膚癌以及皮膚老化中扮演了重要的角色，其中UVB (290~320 nm)會引起DNA斷裂，進而對皮膚造成直接且嚴重的傷害並引起自由基大量產生。自由基誘發細胞內反應性細胞凋亡以及氧化壓力，人類表皮和真皮中的紫外線誘導發炎反應起到了關鍵的作用。蝦紅素(Astaxanthin)是一種葉黃素類胡蘿蔔素，在許多不同器官上皆有益處。在本篇的研究當中，我們發現蝦紅素在體外和體內試驗中會修復紫外光誘導的傷害。在MTT測試中，人類正常角質細胞(HaCaT)以及人類纖維母細胞(HS68)在加入了蝦紅素之後，會降低因UVB照射而引起的細胞死亡。在彗星測試中，DNA會因為照射了UVB而受損並出現拖尾情況，而不同濃度的蝦紅素降低由UVB引起的DNA的傷害，同時我們利用annexinV-FITC/PI雙染確定細胞凋亡情況也會抑制人類正常角質細胞以及人類纖維母細胞凋亡的現象。在機轉探討中，蝦紅素會藉由降低氧化壓力及減低DNA受損，以調降MAPK路徑進而抑制細胞發炎並減緩凋亡的現象。在體內測試的部分，我們使用SD大鼠照射300 mJ誘導光傷害，發現照光後的大鼠會引起背部的紅腫發炎現象，在腹腔注射不同濃度的蝦紅素，蝦紅素會降低受傷老鼠背部皮膚的發炎現象，取下背部的皮膚進行蛋白質表達分析發現，在加入蝦紅素後會降低氧化壓力以及凋亡反應相關蛋白，這些結果說明了蝦紅素會修復紫外光造成的皮膚傷害以及老化藉由抑制發炎以及凋亡反應。

關鍵字

蝦紅素； UVB ；發炎；凋亡；體內測試

並列摘要

UV (Ultraviolet, 200~400 nm) plays a crucial role in inducing skin disorders such as skin cancer and skin aging, in which UVB can cause DNA damage and direct and serious damage to the skin, inducing a large number of free radicals. Free radicals cause inflammatory reactions within the human epidermis and dermis, inducing intra-cellular reactive apoptosis. Astaxanthin (AST) is a xanthophyll carotenoid exhibiting several clinical benefits in different organs. This study investigated the pathway of AST against UV-induced damage in vitro and in vivo. In MTT assay, for human normal keratinocytes (HaCaT) and human normal fibroblasts (HS68), following treatment with AST, cell death induced by exposure under UVB (30 mJ/cm2) was decreased. In comet assay, AST decreased the DNA damage following UVB irradiation. We then used annexin V-FITC/PI stained fluorescence to observe the apoptosis situation of HS68 and HaCaT cells. In the molecular mechanism of AST decrease photo damage, we first found AST decreased oxidative and DNA damage, which then down-regulated the MAPK pathway to inhibit cell inflammation and apoptosis. As for the in vivo test, we exposed SD rats to (300 mJ/cm2) irradiation to induce photo damage, causing redness and inflammation of the back. They were then treated with different concentrations of AST with intraperitoneal injection. Concerning the in vivo investigations, experimental rats in AST treatments following UVB (300 mJ/cm2) irradiation showed decreased oxidative stress and apoptosis injuries in a dose-dependent manner compared to the vehicle control group. These results suggest AST repairs UV induced skin damage and aging by inhibiting the inflammatory reactions and apoptosis responses. Astaxanthin reduced the inflammation of the back skin of injured rats. After examining the skin from the back by analyzing protein expression, it was observed injection of AST reduces oxidative stress and apoptosis-related proteins.

並列關鍵字

astaxanthin (AST) ； ultraviolet irradiation B (UVB) ； skin ； inflammation ； apoptosis ； in vivo test

參考文獻

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Ambati, R. R., Phang, S. M., Ravi, S., & Aswathanarayana, R. G. (2014). Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications—A review. Marine drugs, 12(1), 128-152.

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