新熱敏感型多孔水膠支架之製備與性質

Preparation and Properties of Novel Thermo-Responsive Hydrogels Scaffold

蔡宗憲(Tsung-Hsien Tsai)

指導教授 ： 王志光

高雄醫學大學/生命科學院/醫藥暨應用化學研究所/碩士(2008年)

摘要

本論文旨在開發新型熱敏感型(thermo-responsive)多孔水膠生物支架，並對其作性質的探討。研究中以異丙基丙烯醯胺（N-isopropyl acrylamide , NiPAAm）及甲基丙烯酸（Methylacrylic acid , MAA）為單體，先聚合成P(NiPAAm-MAA)水膠，之後再以聚乙二醇二胺(Polyethylglycol bisamine)為中間交聯劑(cross-linker)將透明質酸(Hyaluronic acid , HA)多醣分子，交聯成P(NiPAAm-MAA)-PEG-HA的複合水膠。此複合新型水膠即結合P(NiPAAm-MAA)較佳機械強度優點，以及透明質酸和聚乙二醇二胺的生物相容性與生物可降解的優勢，聚合出具有三者優點之新熱敏感型多孔水膠生物支架。水膠化學合成物鑑定是以H-NMR、FT-IR、LC-MS等評估，性質探討則是在不同pH值環境下觀察水膠的膨潤變化與含水比例，以黏度儀測試最低臨界溶液溫度(Lower critical solution temperature；LCST)，在體外生理磷酸緩衝液(PBS)浸泡實驗觀察材料降解的情況，並以掃瞄式電子顯微鏡(SEM)觀察凍乾後水膠表面與剖面的多孔結構，最後對材料作細胞毒性評估。初步結果顯示本研究開發的新型熱敏感型多孔水膠之LCST約在34℃、膨潤度在4-8倍、含水率在73-93%，且不具細胞毒性，故在組織再生的支架應用上具有相當潛力。

關鍵字

水膠支架

並列摘要

A new kind of thermo-responsive biological hydrogel scaffolds was synethized in this studies. First, P(NiPAAm-MAA) hydrogels were polymerized with N-isopropyl acryamide (NiPAAm) and Methylacrylic acid (MAA). Then the P(NiPAAm-MAA)-PEG-HA hydrogels were synthesized with Hyaluronic acid (HA) by cross-linking the Polyethyl glycol bisamine (PEG-diamine). The new hydrogles combined both advantages of NiPAAm and HA which including better mechanical properties, biodegradable and biocompatibility. The characteristic of P(NiPAAm-MAA)-PEG-HA hydrogels were evaluated by H-NMR, FT-IR and Viscometer. The swelling ratio and water content of hydrogels were measured in different pH value at 37℃. And the lower critical solution temperature (LCST) of the hydrogels was measured with different temperature by Viscometer. The hydrogels were immersed in PBS (pH=7.4) at 37℃ to observed the change of weight lost. The surface of hydrogels after freeze-drying were observed by SEM. Finally, MTS activity assay of hydrogels cultured for 24, 48, 72 hours. The results showed that the hydrogels exhibited a LCST at 34℃ and swelling ratio was between 4-8 times and water content was between 73-93%. The swelling ratio and water content depended on the pH value of PBS solution. MTS activity culture prove that the hydrogels were no toxicity. So The new thermo-responsive P(NiPAAm-MAA)-PEG-HA hydrogels will have great potential applications in tissue engineering.

並列關鍵字

Hydrogels scaffold

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