放射性碘去氧尿嘧啶之合成:應用於SH-SY5Y細胞吞噬後經X-光照射之過氧化研究

The sysnthesis of radioactive IUdR:A preliminary study of the peroxidation of SH-SY5Y cell uptake IUdR with X-ray irradiation

陳囿霖(Yo-Lin Chen)

指導教授 ： 鍾相彬

高雄醫學大學/健康科學院/醫學影像暨放射科學系碩士班/碩士(2015年)

摘要

此研究為探討IUdR與X-ray照射，對SH-SY5Y細胞產生之影響。本實驗使用合成之放射性碘去氧尿嘧啶131IUdR，檢測SH-SY5Y細胞之吞噬率，並以SH-SY5Y細胞作為神經元模型，使用IUdR 做為輻射增敏劑再照射X-ray，並測試不同條件對細胞所造成的影響。細胞實驗選定添加使用劑量為100 µM 的IUdR與X-ray照射 2 Gy，來做為探討氧化作用對細胞產生的影響劑量，藉由MDA濃度檢測與ROS氧化檢測，來探討氧化影響對細胞蛋白質的變化。實驗結果顯示，細胞添加 IUdR或照射X-ray，確實會造成MDA與ROS濃度明顯上升，再比較X-ray照射組與IUdR+X-ray組，可發現兩組的MDA濃度相近，但針對ROS濃度所做實驗，可測得IUdR+X-ray組之ROS濃度明顯高於X-ray照射組。因此添加IUdR與照射X-ray會促進ROS濃度上升及相對提高MDA濃度，並利用以上研究結論推測ROS或可活化MAPK、PI3K/Akt等路徑來刺激細胞分裂的訊息傳遞，因而促進SH-SY5Y細胞增生。期望本研究之結論可為未來神經退化疾病等研究提供參考之依據。

關鍵字

放射性碘去氧尿嘧啶 ； 活性氧物質 ； 脂質過氧化

並列摘要

This paper was indicated the effects of IUdR and X-ray to SH-SY5Y cells.
This experiment was utilized radioactive 131IUdR to measure the cell uptake rate. The IUdR was used as the radiosensitizer and following the exposure of X-ray. After treated with 100 µM of IUdR and 2 Gy of X-ray, the concentrations of MDA and ROS oxidation were measured by ELISA and Chemiluminescence detection method. The experimental results showed that the IUdR and X-ray were increased MDA and ROS concentrations significantly. In conclusion, the IUdR and X-ray will encourage ROS concentration and relative MDA concentration. The ROS may induce the activation of MAPK and PI3K/Akt pathways to enhance cell division. It may provide the references for future research in neurodegenerative disease.

並列關鍵字

5-iodo-2-deoxyuridine ； Reactive oxygen species ； Malondialdehyde

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