以蛋白質體學探討材料表面改質對纖維母細胞影響之研究

Assessing the response of fibroblast to material surfaces by proteomics approaches

徐可欣(Ko-Hsin Hsu)

指導教授 ： 鍾相彬

高雄醫學大學/健康科學院/醫學影像暨放射科學系碩士班/碩士(2011年)

https://doi.org/10.6832/KMU.2011.00077

摘要

材料表面改質在現今的組織工程是非常熱門的一個階段，在材料表面改質方面可以用於替代器官的發展等生醫材料上。現今有很多的材料來做表面改質，如甲殼素(Chitosan, CS)、玻尿酸(Hyaluronic Acid, HA)及蠶絲蛋白(Silk Fibroin, SF)。本實驗是以奈米碳管(Carbon nanotubes, CNT)作為底層以增加材料表面積與細胞的作用。利用石英晶體微天平(quartz crystal microbalance, QCM)來測試細胞貼附的情況，並將所收集好的細胞以質譜儀來分析及鑑定細胞貼附於材料表面後是否有不同之蛋白質身分。實驗結果在石英晶體微天平部份可以測得在以奈米碳管/甲殼素/玻尿酸(CNT/CS/HA)的材料表面細胞之生長及貼附情況比只有奈米碳管(CNT)的材料表面佳，以質譜儀來分析及鑑定蛋白質身分，結果總共鑑定出有五種蛋白質是與一般的纖維母細胞不同的蛋白，包括了CD44 antigen, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Cell division protein kinase 9, Platelet glycoprotein 4, Nucleophosmin等五種蛋白，這些蛋白的作用都與細胞的吸附，細胞增生等有關，可以了解到材料表面改質是會讓細胞產生更容易吸附及增生的蛋白質，了解釋放出不同之蛋白質以利細胞的貼附及生長，期望能找出對於細胞吸附更有效的材料表面。

關鍵字

蛋白質體學 ； 石英晶體微天秤 ； 生醫材料

並列摘要

Surface modification in tissue engineering is a very popular stage now. The qualitative change in the surface can be used to replace organs such as the development of biomedical materials. There are a lot of material for the surface modification, such as chitin ( Chitosan, CS), hyaluronic acid (Hyaluronic Acid, HA) and silk protein (Silk Fibroin, SF). This experiment is based on carbon nanotubes (Carbon nanotubes, CNT) as the underlying material to increase the surface area for cell adhesion. We used quartz crystal microbalance (quartz crystal microbalance, QCM) to test the cell attached, and the collected cells to the mass spectrometer to analyze and identify the proteins of the cell attached on the different surface have different identity. The results in the QCM can be measured the cell growth and adhesion of carbon nanotubes / chitosan / hyaluronic acid (CNT / CS / HA) better than only carbon nanotubes(CNT). The mass spectrometry to analyze and identify protein identity, the results of total protein was identified in normal fibroblast cells with five different proteins, including CD44 antigen, Serine / threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Cell division protein kinase 9, Platelet glycoprotein 4, Nucleophosmin. The five proteins, the role of these proteins with the cell adsorption, cell proliferation. Because of the related materials and surface modification, we can learn to make the cells more easily absorbed and proliferation protein, a different interpretation of release to facilitate cell adhesion proteins and growth, hoping to find more effective for cell adhesion surface.

並列關鍵字

proteomics ； QCM ； biomedical material

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