建立C1~C10醛類物質的採樣分析方法以評估油煙中醛類物質的濃度分佈之研究

Establishment of C1~C10 aldehdye sampling and analysis method for determining aldehyde concentration distribution in cooking oil fumes

翁昭枝(Chao-Chih Weng)

指導教授 ： 彭瓊瑜

高雄醫學大學/健康科學院/職業安全衛生研究所/碩士(2010年)

https://doi.org/10.6832/KMU.2010.00096

摘要

廚房油煙中含有相當多的化學物質，其中醛類物質是油煙中特徵排放物，長鍊的醛類物與油脂中的脂肪酸氧化相關；除此之外，有些醛類物質為致癌物或疑似致癌物，量測油煙中的醛類物質為一重要的研究課題，然而，目前針對油煙中醛類物質完整的量測方法闕如，因此本研究目的是建立有效而方便的C1~C10醛類物質的氣粒狀採樣分析方法，同時探討採樣介質之回收效率，及實際廚房環境的應用，以評估油煙中醛類物質的濃度分佈，了解暴露油煙的相關資料。
本研究使用DNPH-裹附的玻璃濾紙串聯DNPH吸附管以採集油煙中粒相、與氣相的醛類物質，樣本以高效能液相層析儀/紫外可見光偵測儀分析。實驗室的評估結果顯示此方法的線性範圍0.995 ~ 0.999 (R2)，偵測下限為0.002 ~ 0.014μg/mL，再現性為2.37% ~ 6.78% (CV%)，脫附效率為80% ~ 100%，皆符合環境偵測的規範；實際採樣上，發現此方法適用於廚房油煙的樣分析。

關鍵字

油煙 ； 醛類物質

並列摘要

There exist a lot of chemicals in cooking oil fumes (COFs), especially long-chain aldehydes, which are frequently found in COFs and exclusively in oil-related emissions. It is important to measure the emissions of aldehydes, such as trans,trans-2,4-decadienal which is a potential tumor promoter and can be served as a characteristic compound of COFs. Few studies have investigated aldehyde distributions of COFs comprehensively; therefore, this study establishes a C1~C10 aldehyde sampling and analysis method for both particulate- and gas phases, and applies this method to evaluate concentration distribution of aldehydes in COFs.
The cooking oil fumes were collected using DNPH-coated glass fiber filters (25 mm) and 2,4-DNPH cartridges (47285-U SUPERLCO) connected in serial for measuring particulate- phase and gas-phase aldehydes, respectively. After sampling, samples were analyzed by HPLC/UV in gradient mode. This method was optimized for 20 carbonyl chemicals analysis. This method was evaluated in terms of linearity, method detection limit, reproducibility and desorption efficiency in the laboratory. After laboratory evaluation, this method was applied in five kitchens for field assessment. Results show that linearity, method detection limit, reproducibility (expressing as CV) are 0.995-0.999 (R2), 0.002-0.014 μg/mL and 2.37-6.78 % (CV), respectively. The recovery rates of 2,4-DNPH cartridges are 80 ~ 100% for concentration range between 0.2678- 0.3349 μg/ml. They are within in the acceptable range. This method also shows applicable for field test.

並列關鍵字

cooking oil fumes ； aldehydes

參考文獻

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