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  • 學位論文

不同選擇性培養基在變異型鏈球菌族群裡的檢測效率、選擇性與鑑別性之比較

Comparisons of Selective Media for the Recovery of Streptococcus mutans: Differentiation Reliability and Recovery Rate

指導教授 : 黃純德

摘要


要形成齲齒有一個必要的因素就是細菌的存在,而最主要引起齲齒的細菌為變異型鏈球菌(Streptococcus mutans),在以往的研究中曾使用各種的選擇性培養基,來篩選口內的變異型鏈球菌,期望能夠使用最簡易且最具選擇性(selective)的方式,來得到所要的變異型鏈球菌。本實驗選取近幾年來被認為較有效的四種選擇性培養基(MSB、TSY20B、GSTB與MSKB)作測試,比較它們的選擇性(selective)、檢測效率(Recovery rate)以及菌落型態的鑑別能力(differentiation reliability),結果發現MSB選擇性培養基較其他三種適合用來檢測人類齲齒病原菌,尤其是針對Streptococcus mutans與Streptococcus sobrinus兩種菌。

並列摘要


Caries is mainly caused by the presence of bacterial plaque. Numerous studies have shown that Streptococcus mutans group plays an important role in causing dental caries. The objective of this study is to compare the efficacy and reliability of four well-recognized selective and differential media (namely: MSB、TSY20B、GSTB and MSKB) for Streptococcus mutans group bacteria. The result of this study shows that MSB is the most suitable use in detecting cariogenic Streptococcus mutans and Streptococcus sobrinus based on their reliability in differentiation reliability and recovery rate.

並列關鍵字

Caries Streptococcus mutans Selective media

參考文獻


1.Clarke JK. On the bacterial factor in the aetiology of dental caries.British J Exp Pathol 1924; 5: 141-147.
2.Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST, eds.Bergey's manual ofdeterminative bacteriology. 9th ed. Williams and Wilkins: Baltimore, Maryland 1994; pp. 20 and 527-558.
3.Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998; 13: 195-216.
4.Maiden MFJ, Lai C-H, Tanner A. Characteristics of oral gram-positive bacteria. In: Contemporary Oral Microbiology and Immunology. Slots J, Taubman MA, eds. Mosby: St. Louis, MI 1992; pp. 342-372.
5.Carlsson P. On the epidemiology of mutans streptococci. Thesis, Malmo: University of Lund, 1988.

被引用紀錄


Yang, B. C. (2011). 台灣國際觀光旅館經營績效的決定因素 [master's thesis, Yuan Ze University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0009-2801201414590453
劉瀚升(2011)。探討台灣品牌經營成功關鍵因素〔碩士論文,亞洲大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0118-1511201215471833

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