利用生物資訊學的分析來找尋疾病相關基因之研究

隨著人類基因體計畫的完成以及高效能生物技術的發展，生物資訊學已成為生物醫學研究發展中不可或缺的學門。本研究目的在於利用生物資訊學分析，進行動脈粥狀硬化與近視的疾病易感基因之分析及確定。在第一部分的研究中，找尋動脈粥狀硬化之致病機轉中，由氧化型低密度脂蛋白誘發的微小核醣核酸所調控之基因變化。利用軟體分析預測其標靶基因並以實驗證實之。在第二部分的研究中，利用生物反應路徑分析來找尋近視的易感性基因。其後，挑選出一侯選基因進行實驗並加以驗證其參與疾病之過程。由本研究結果得知，利用生物資訊學的分析可為我們現有研究提供新的方向。

關鍵字

生物資訊；動脈粥狀硬化；近視

並列摘要

With the completion of the Human Genome Project and the development of high-throughput experimental technologies, bioinformatics has become an essential part of research and development in the biomedical sciences. The purpose of this dissertation was to identify the susceptibility genes of atherosclerosis and myopia using in silico analysis. In the first part of this study, we sought to identify target genes of microRNA that are involved in oxLDL-stimulated atherosclerosis formation. We used computational target prediction programs to compile the putative microRNA target genes. In the second part of this study, we investigated genetic loci influencing susceptibility to myopia. We conducted pathway analysis to select promising genes from our genome wide association study of myopia. Subsequently, we selected one candidate gene for further validation study. This study may provide new insights into the research areas we have previously worked on.

並列關鍵字

bioinformatics ； atherosclerosis ； myopia

參考文獻

Ambros, V. (2004). "The functions of animal microRNAs." Nature 431(7006): 350-355.

Google Scholar

Ashburner, M., C. A. Ball, et al. (2000). "Gene ontology: tool for the unification of biology. The Gene Ontology Consortium." Nature Genetics 25(1): 25-29.