- 學位論文
水筆仔衍生菌化學成分研究
Chemical Constituents of the Fungus Diaporthe arecae Derived from Kandelia obovata
摘要
「紅樹林」指的是生長在陸地與海洋交界處的沿海灌木群，主要分布在熱帶與亞熱帶地區。紅樹林的生態系統非常特別，例如高濕度、高鹽度、低含氧量、薄泥土層和潮汐。但就是因為這些嚴苛的環境，孕育了許多特殊的植物、動物與微生物，其中又以耐鹽微生物與嗜鹽微生物最為特別。紅樹林內生菌被認為是一個天然物的大寶庫，引起了天然物化學家們極大的研究興趣。 從六種台灣紅樹林的植物內取出了二十種內生菌，並對這些真菌的乙酸乙酯層粗萃物進行活性篩選評估。發現水筆仔(Kandelia obovata)中分離出的內生真菌間座殼菌(Diaporthe arecae)其粗萃物對人類肝癌、肺癌與乳癌(HepG2, A549, MDA-MB-231)細胞株具有顯著的細胞毒殺活性。因此我們進行了一系列的色層分析實驗，分離純化出了一個新吲哚甘油類化合物1和二十三個二胜肽類化合物2-24。所有化合物的結構是依據其質譜儀(MS)和核磁共振儀(NMR)等數據決定。並發現化合物6具有抗血管新生之活性，可抑制管狀內皮細胞的生成，IC50值為15.2 μg/mL。
並列摘要
Mangrove is littoral shrub distributes worldwide in subtropics and tropics. The mangrove ecosystem is a high humidity, high salinity, and hypoxia tolerant ecosystem which supports the existence of many halotolerant and halophilic microorganisms. Mangrove-derived fungi are regarded as abundant sources of bioactive natural products and have aroused great interests of natural product chemists. In our screening of Taiwanese mangrove-derived fungi, 20 species of mangrove-derived fungi were successfully purified and cultured. The EtOAc extracts of all isolated fungi were evaluated for anti-cancer activity. As a result, the EtOAc extract of Diaporthe arecae isolated from Kandelia obovata showed potent cytotoxic activity against A549, HepG2, and MDA-MB-231 human cancer cell lines. Accordingly, a series of bioassay-guided for this fungus was carried out. After repeated chromatography, one new indoleglycerol (1) and 23 diketopiperazines (2−24), were isolated from the culture broth of D. arecae. The structures of all isolates were determined by their mass and NMR (COSY, HMBC, and HSQC) spectroscopic data. In the anti-angiogenic assay, cordysinin A (6) displayed mild inhibited effect by suppressing tube formation with an IC50 value of 15.2 μg/mL.