香菸和二手菸暴露與血中環氧乙烷鍵結物濃度之相關研究

Association between active and passive smokers and N-(2-hydroxyethyl)valine levels in hemoglobin

涂瑋真(Wei-Chen Tu)

指導教授 ： 吳明蒼

高雄醫學大學/健康科學院/職業安全衛生研究所/碩士(2006年)

https://doi.org/10.6832/KMU.2006.00115

摘要

目的：研究抽菸、二手菸暴露對人體血紅素形成之環氧乙烷共價鍵結物(N-hydroxyethylvaline；HEV)濃度之相關性。分別探討女性與男性體內HEV值與抽菸和二手菸暴露之相關性。

方法：本研究之女性研究對象(n=78)是由子宮頸前癌與子宮頸癌之流行病學研究中，以有抽菸婦女(n=14)和無抽菸(n=32)及二手菸暴露(n=32)之婦女配對(年齡±3歲)。而男性部分(n=110)是由健檢資料庫中，選取有抽煙者(n=55)和無抽菸者(n=55)配對(年齡±3歲)作為研究對象。利用問卷調查的方式，收集其基本資料、抽菸習慣；另外收集血液樣本。血液樣本經過前處理之後，採用modified Edman degradation method 進行HEV濃度之測定。

結果：男性抽菸者(n=55)體內的HEV值為109.4 pmol/g Hb與非抽菸者(n=55)體內HEV值為53.6 pmol/g Hb，達統計上顯著意義(p<0.001)；而女性抽菸者(n=14)體內HEV值為219.4 pmol/g Hb、二手菸暴露者(n=32) HEV值為53.2 pmol/g Hb和非抽菸者體內HEV值為40.9 pmol/g Hb，亦達統計上顯著意義(p<0.001)。結果與過去文獻相符，抽菸者體內之HEV值，不論性別，皆會顯著高於非抽菸者2~3倍多。

結論：從本研究的結果得知抽菸者體內HEV值確實高於非抽菸者與二手菸暴露者，故HEV值為評估因抽菸而暴露到環氧乙烷之良好生物指標。

關鍵字：環氧乙烷環氧乙烷血中鍵結物、抽菸、性別、GC/MS/MS。

關鍵字

環氧乙烷 ； 環氧乙烷血中鍵結物 ； 抽菸 ； 性別 ； GC/MS/MS

並列摘要

Objective : The purpose of this thesis is to investigate the association between cigarette smoking and ethylene oxide-induced hemoglobin adducts (N-hydroxyethylvaline, HEV). And confer the correlation of gender and smoking habit with the HEV concentration in human hemoglobin.
Methods : In this study , females(n=78) was from the molecular epidemiologic study of cervical intraepithelial neoplasia and invasive cervical cancer and males was from a physical examination center. No-smokers and second-hand smokers were matched to the smokers by age (±3 years). To collect the information about smoking habits by questionnarires. And also collected the blood samples. Then use modified Edman degradation method to analyze ethylene oxide-induced hemoglobin adducts.
Result : The amounts of male’s HEV are 109.4 pmol/g Hb in smokers and 53.6 pmol/g Hb in no-smokers which are significantly different(p<0.001). The amounts of female’s HEV are 219.4 pmol/Hb in smokers and 47.1 pmol/Hb in no-smokers which are significatly different(p<0.001). The results are the same with references, the amounts of HEV in smokers are higher concentration than no-smokers.
Discussion: This study validates that HEV is a sensitive and specific biomarker to assess ethylene oxide exposures from tobacco smoke.

Key words: ethylene oxide, N-hydroxyethylvaline, tobacco smoke, gender, GC/MS/MS.

並列關鍵字

ethylene oxide ； N-hydroxyethylvaline ； tobacco smoke ； gender ； GC/MS/MS

參考文獻

1. L. Hagmar, Z. Mikoczy, and H. Welinder, "Cancer incidence in Swedish sterilant workers exposed to ethylene oxide," Occup Environ Med 52(3), 154-156 (1995).

2. S. S. Wang and J. M. Samet, "Tobacco smoking and cancer: the promise of molecular epidemiology," Salud Publica Mex 39(4), 331-345 (1997).

3. M. Törnqvist, S. Osterman-Golkar, A. Kautiainen, S. Jensen, P. B. Farmer, and L. Ehrenberg, "Tissue doses of ethylene oxide in cigarette smokers determined from adduct levels in hemoglobin," Carcinogenesis 7(9), 1519-1521 (1986).

4. A. D. Tates, T. Grummt, M. Tornqvist, P. B. Farmer, F. J. van Dam, H. van Mossel, H. M. Schoemaker, S. Osterman-Golkar, C. Uebel, Y. S. Tang, and et al., "Biological and chemical monitoring of occupational exposure to ethylene oxide," Mutat Res 250(1-2), 483-497 (1991).

5. S. Osterman-Golkar and E. Bergmark, "Occupational exposure to ethylene oxide. Relation between in vivo dose and exposure dose," Scandinavian Journal of Work, Environment & Health 14(6), 372-377 (1988).