利用化學計量實驗設計法搭配微胞崩解線上濃縮毛細管電泳法進行化粧品中化學性防曬成分之分析研究

Application of experimental design by micelle collapse on-line preconcentration capillary electrophoresis for the determination of ultraviolet absorbents in sunscreen products

蕭文瑤(Wen-Yao Hsiao)

指導教授 ： 陳彥伶

高雄醫學大學/藥學院/香粧品學系碩士班/碩士(2014年)

摘要

本研究使用毛細管電泳儀結合微胞崩解分析物聚集搭配膠束電泳毛細管層析法及化學計量實驗設計法偵測化粧品中十種紫外線吸收劑。此濃縮技術藉由待測物與微胞之間的傳遞、釋放及濃縮的特性來達到樣品堆積以及分離目的。化學計量實驗設計法目的為獲得最佳化分離條件其包括兩水準部分因子實驗設計法(fFD)及中心混成實驗設計法(CCD)，相較傳統的因子探討，能減少時間與成本的損耗。其檢量線之相關係數均大於0.995，RSD值與RE值均小於9.94%，代表此分析方法具有良好的直線性、準確度及精密度。此分析方法已成功應用於數種市售化粧品的含量檢測。

關鍵字

實驗設計法 ； 紫外線吸收劑 ； 線上樣品縮技術 ； 微胞崩解分析物聚集搭配膠束電泳毛細管層析法

並列摘要

In order to prevent UV damage to the skin, many sunscreen products that can absorb UV radiation and attenuate the negative effects of sunlight exposure were developed. However, ultraviolet absorbents have some adverse reactions such as skin irritation. On- line sample concentration technique, analyte focusing by micelle collapse-micellar electrokinetic capillary chromatography (AFMC-MEKC), was introduced to improve detection sensitivity. The aim of this study is to establish an on- line preconcentration method which can simultaneously determine these ultraviolet absorbents and their metabolites in plasma and urine samples. The chemometric experimental design including fractional factorial design (fFD) and central composite design (CCD) were used to optimize separation conditions in this study. Compared to the traditional investigation, the chemometric experimental design was less time-consuming, lower in cost and can explore the interactions of parameters. The influences of three experimental variables (pH value of buffer, SDS concentration and organic solvent percentage) on the response were investigated by applying a chromatographic exponential function. After optimization, this AFMC-MEKC method was considered to be established and was successfully applied to several commercial sunscreen products.

並列關鍵字

Experimental design ； Ultraviolet absorbents ； On-line sample preconcentration ； Analyte focusing by micelle collapse-micellar electrokinetic capillary chromatography.

參考文獻

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