光學影像基質金屬蛋白酶3 (MMP-3)胜肽
、DOTA-RGD及TTDA-ITCP-BN
之合成研究

本研究開發合成Matrix Metalloproteinase 3 (MMP-3) 胜肽，及其控制組胜肽。利用HPLC純化，再經過質譜儀鑑定結果，其分子量為1842.36 g/ mol，與實際分子量1842.13 g/ mol相符合。在此設計MMP-3 peptide序列，主要是由於MMP-3酵素會針對其Glu-Nval殘基進行切割，而控制組胜肽則否，藉此偵測MMP-3酵素。此外本研究又針對了Bombesin及KGRGD序列，分別合成了DOTA-RGD及TTDA-ITCP-BN， 2個錯合物。其中KGRGD peptide序列利用HPLC純化，經過質譜儀鑑定結果，其分子量為754.29 g/ mol，與實際分子量754.10 g/ mol相符合; 而BN(7-14) peptide序列同樣經過HPLC純化，利用質譜儀鑑定結果，其分子量為1162.55 g/ mol，與實際分子量1162.30 g/ mol相符合。此外，也成功的合成出TTDA-ITCP，利用核磁共振(Nuclear Magnetic Resonance)及質譜儀鑑定，結果相符合。

關鍵字

光學影像基質金屬蛋白酶3

並列摘要

Matrix Metalloproteinase 3 (MMP-3) and control peptide substrate was designed. To employ HPLC purify, and use mass spectrometer to appraise result. It molecular weight is 1842.36 g/ mol, and intrinsical molecular weight is 1842.13 g/ mol. We designed MMP-3 peptide substrate, as a result of MMP-3 enzyme had cleave Glu-Nval, however control did not . We had aim Bombesin and KGRGD substrate respectively conjugate with DOTA-RGD and TTDA-ITCP-BN. KGRGD peptide substrate had employ HPLC purify, and use mass spectrometer to appraise result. It molecular weight is 754.29 g/ mol, and intrinsical molecular weight is 754.10 g/ mol. BN(7-14) peptide substrate had employ HPLC purify, and use mass spectrometer to appraise result. It molecular weight is 1162.55 g/ mol, and intrinsical molecular weight is 1162.30 g/ mol. In addition, we also designed TTDP-ITCP successful. And had employ NMR (nuclear magnetic resonance ) and mass spectrometer to assay that.