懷孕婦女牙周狀況與牙齦溝液PGE2、IL-1β、TNF-α和血液中PGE2、TNF-α含量之相關性

The relationships among periodontal conditions, the levels of PGE2、IL-1β、TNF-α in gingival crevicular fluid and the levels ofPGE2、TNF-α in peripheral blood of pregnant women

侯玫妃(Mei-Fei Hou)

指導教授 ： 蔡吉政 ； 洪信嘉

高雄醫學大學/口腔醫學院/牙醫學研究所/碩士(2004年)

摘要

目前已有許多研究證實孕婦的牙周病是造成體重不足早產兒的一大危險因子，也有許多假設推論牙周病造成體重不足早產兒發生的機轉，但是目前並沒有一個定論。本研究針對這樣的疑問，收集同意參加本研究的孕婦前後孕期(第一、第三孕期或第二、第三孕期)之牙齦溝液及血漿檢體，以ELISA檢測自西元2003年9月1日至西元2004年3月30日為止共收集到的33名孕婦的牙齦溝液及血漿中之PGE2、IL-1β和TNF-α，並分析兩兩之間的關係。研究結果發現孕婦的臨床牙周指數與牙齦溝液中的PGE2(Mean±SD: 101.0±73.8 ng/ml, 89.7±26.5 pg/30s)、IL-1β(29.1±29.9 ng/ml, 36.9±29.3 pg/30s)、TNF-α(158.4±131.4 pg/ml, 0.116±0.102 pg/30s)濃度和總量及血漿中的PGE2(2.18±1.74 ng/ml)、TNF-α(1.07±0.556 ng/ml)濃度沒有顯著相關(p > 0.05)；血漿中的PGE2與收集30秒牙齦溝液中的PGE2、IL-1β、TNF-α的濃度、總量也沒有顯著相關(p > 0.05)；血液中TNF-α的濃度和牙齦溝液中PGE2、IL-1β和TNF-α的濃度沒有相關(p > 0.05)，但是當我們使用收集30秒牙齦溝液樣本中的PGE2、IL-1β和TNF-α的總量來進行分析發現血液中TNF-α的濃度和牙齦溝液中PGE2(r = 0.424, p = 0.0052)、IL-1β(r = 0.333, p = 0.0385)和TNF-α(r = 0.483, p = 0.0038)的總量是成顯著正相關的(p < 0.05)。本研究發現血液中TNF-α的濃度和牙齦溝液中的PGE2、IL-1β和TNF-α可能是有關的，它們之間是否有因果關係，還需要進一步的研究來探討。

關鍵字

體重不足早產兒 ； 牙周病 ； 牙齦溝液 ； PGE2 ； IL-1β ； 細胞激素 ； 血漿 ； TNF-α ； 血液

並列摘要

Many studies have reported the association between periodontal disease and preterm low birth weight (PLBW) infants. Several pathways have proposed to link the periodontal disease to the occurrences of PLBW infants. However, the evidences are still limited to clarify the mechanisms. Our study collected the plasma and the gingival crevicular fluid (GCF) samples at early and late trimesters (1st and 3rd trimester or 2nd and 3rd trimesters) of pregnant women and examined the relations among results of clinical periodontal examinations, the levels of PGE2, IL-1β and TNF-α in the plasma and the levels in the GCF of the 33 pregnant women. We found that the periodontal clinical parameters of these pregnant women were not associated with the concentration and the total amount of PGE2(Mean±SD: 101.0±73.8 ng/ml, 89.7±26.5 pg/30s), IL-1β(29.1±29.9 ng/ml, 36.9±29.3 pg/30s) and TNF-α(158.4±131.4 pg/ml, 0.116±0.102 pg/30s) in 30-seconds GCF samples, and the concentration of PGE2(2.18±1.74 ng/ml) and TNF-α(1.07±0.556 ng/ml) in plasma (p > 0.05). There was no significant relation between the concentration and the total amount of PGE2, IL-1β and TNF-α in 30-second GCF samples and the concentration of plasma- PGE2 (p > 0.05). The concentration of GCF-PGE2, GCF-IL-1β(r = 0.333, p = 0.0385) and GCF-TNF-α(r = 0.483, p = 0.0038) were not associated with plasma-TNF-α (p > 0.05), but the total amount of PGE2(r = 0.424, p = 0.0052), IL-1β and TNF-α in 30-second GCF sample were significantly positively associated with the concentration of plasma-TNF-α (p < 0.05). Our study found that the levels of GCF-PGE2, GCF-IL-1β and GCF-TNF-α were related to the concentration of blood-TNF-α. Further studies are needed to clarify the casual association.

並列關鍵字

PLBW ； preterm low birth weight ； periodontitis ； gingival crevicular fluid ； GCF ； PGE2 ； TNF-α ； blood ； IL-1β ； plasma ； cytokine

參考文獻

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