台灣前胡根部化學成分及抗血小板凝集活性之研究

Studies on Chemical Constituents and their Anti-platelet Aggregation Activity from the Root of Peucedanum formosanum Hayata

陳鵬印(Peng-Yin Chen)

指導教授 ： 陳益昇

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2004年)

摘要

中文摘要

前胡屬台灣特有種台灣前胡 (繖形科) 是多年生草本植物，主要分佈於本島中高海拔山區，因為與中藥前胡同屬，其根部為傳統民間用藥，具有解熱、鎮咳、鎮痛、袪痰等作用。由於篩選其根部甲醇萃出物發現具有抗血小板凝集活性，故這次研究是採集台灣前胡之根部，進行成分分離，且研究分離化合物之抗血小板凝集活性。
從台灣前胡甲醇萃取液之不可溶部分，獲得到大量D-mannitol (1)，而氯仿可溶部分，則分離得到31個巳知化合物。包括25個coumarin類: bergapten (2)，isoimperatorin (3)，(–)-deltoin (4)，xanthotoxin (5)， praeruptorin E (8)，hyuganin A (9)，(–)-peujaponisin (10)，(–)-isosamidin (11)，(+)-peuformosin (12)，(+)-anomalin (13)，(+)-cis-3’-acetoxy-4-(2-methylbutyroyloxy)-3’,4’-dihydroseselin (14)， cis-3’-hydroxy-4’-isovaleryloxy-3’,4’-dihydroseselin (15)，laserpitin (16)，(–)-cis-3’-hydroxy-4’-(2-methylbutyroyloxy)-3’,4’-dihydroseselin (17)，(+)-marmesin (18)，isoscopoletin (19)，umbelliferone (20)， psoralen (24)， (+)-lomatin (25)，isofraxidin (26)，(–)-cis-khellactone (27)， (+)-3’-hydroxymarmesin (28)，(+)-rutaretin (29)，(+)-oxypeucedanin hydrate (30)，(+)-dorsteniol (31)；1 個monoglyceride 之1-O-hexadecanoyl glycerol (32)，固醇類β-sitosterol (6) 和stigamasterol (7) 之混何物；2個polyacetylene類之 falcarindiol (22)，panaxynol (23) ；1個酚類 p-hydroxyphenethyl ferulate (21)。其結構依光譜數據而決定。
化合物 (2)、(3)、(4)、(5)、(18)、(20)、(24)、(26) 及 (30) 為本科室自其他植物分離到具有抗血小板凝集活性，而 (7)、(22)、(23) 及 (27) 由文獻亦巳知具有此活性。(11)、(12)則對collagen 誘導之抗血小板凝集顯現活性。

關鍵字

台灣前胡 ； 繖形科 ； 抗血小板凝集活性

並列摘要

Abstract
Peucedanum formosanum Hay. (Umbelliferae) is an endemic perennial herb in Taiwan, distributes at medium to high altitudes through the Island. Its root was used as folk medicine to treat cough, fever, headache and sputum caused by colds like the traditional Chinese medicine, Qain Hu. The methanolic extract of the root showed anti-platelet aggregation activity in preliminary screening. Investigation on the MeOH extract of the root led to the isolation of one saccharide, D-mannitol (1) from theparcipitate portion, thirty-one known compounds including coumarins bergapten (2), isoimperatorin (3), (–)-deltoin (4), xanthotoxin (5), praeruptorin E (8), hyuganin A (9), (–)-peujaponisin (10), (–)-isosamidin (11), (+)-peuformosin (12), (+)-anomalin (13), (+)-cis-3’-acetoxy-4-(2-methylbutyroyloxy)-3’,4’-dihydroseselin (14), cis-3’-hydroxy-4’-isovaleryloxy-3’,4’-dihydroseselin (15), laserpitin (16), (–)-cis-3’-hydroxy-4’-(2-methyl-butyroyloxy)-3’,4’-dihydroseselin (17), (+)-marmesin (18), isoscopoletin (19), umbelliferone (20), psoralen (24), (+)-lomatin (25), isofraxidin (26), (–)-cis-khellactone (27), (+)-3’-hydroxymarmesin (28), (+)-rutaretin (29), (+)-oxypeucedanin hydrate (30), and (+)-dorsteniol (31); one monoglyceride, 1-O-hexadecanoyl glycerol (32); mixture of β-sitosterol (6) and stigamasterol (7); two polyacetylenes falcaridiol (22), and panaxynol (23); one benzenoid, p-hydroxyphenethyl ferulate (21). The structures of isolates were elucidated by spectroscopic analysis.
Among the isolates, compounds (2), (3), (4), (5), (18), (20), (24), (26) and (30) with anti-platelet aggregation activity were previously reported by our lab. The anti-platelet aggregation activity of (13), (22), (23) and (27) were also reported by other groups. Compouds (11) and (12) showed anti-platelet aggregation activity induced by collagen.

並列關鍵字

Umbelliferae ； Peucedanum formosanum ； anti-platelet aggregation activity

參考文獻

參考文獻

1. 邱年永，張光雄，原色台灣藥用植物圖鑑，南天書局，台北，1992； Vol. III: p.153.

2. Kao MT. Umbelliferae in Flora of Taiwan, 2nd. ed. Editorial Committee of the Flora of Taiwan, Taipei, Taiwan. 1993; Vol. III: p.1010-45.

3. 顏焜熒，常用中藥之藥理，國立中國醫藥研究所，台北，1970；Vol. II: p 54-59.

4. Chen IS, Chang CT, Sheen WS, Teng CM, Tsai IL, Duh CY and Ko FN. Coumarins and antiplatelet aggregation constituents from Formosan Peucedanum japonicum. Phytochemstry 1996; 41: 525-30.