毛細管電泳微胞電動層析法對藥物於製劑與生物檢體中之研究與應用

Analysis of drugs in drug formulations and biological sample with micellar electrokinetic chromatography

葉欣樺(Hsin-Hua Yeh)

指導教授 ： 陳素惠

高雄醫學大學/藥學院/藥學研究所/碩士(2005年)

摘要

本論文乃利用毛細管電泳法之微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC），分別針對不同的藥物，發展簡單且快速的分析方式。主要的研究包括：針對aminoglycoside類抗生素中amikacin、kanamycin A及tobramycin之製劑分析，以及人體血漿及腦脊髓液中促進腦部血液循環藥piracetam之分析。主要的成果摘錄如下：

1、 毛細管電泳法對aminoglycoside類抗生素amikacin、kanamycin A及tobramycin之同時分析及其市售製劑應用(Electrophoresis, 2005, 26, 947-953.)

本論文針對amikacin、kanamycin A及tobramycin建立一種簡單的微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC）之分析。此分析之background electrolytes為：含陰離子界面活性劑300 mM sodium pentanesulfonate 之180 mM Tris緩衝溶液（pH 9.1）。影響分離的參數包括：緩衝溶液的濃度與pH值、以及sodium pentanesulfonate的濃度。此分析條件可應用於amikacin、kanamycin A及tobramycin之製劑分析。
2、毛細管電泳法對血漿及腦脊髓液中piracetam之分析研究

本論文建立一種簡單的微胞電動層析法（Micellar Electrokinetic Chromatography，MEKC），可針對血漿及腦脊髓液中piracetam進行定量分析研究。此分析之background electrolytes為：含280 mM sodium dodecyl sulfate（SDS）之30 mM Tris緩衝溶液（pH 9.5）。影響分離的參數包括：緩衝溶液的濃度與pH值、以及SDS的濃度。此分析方法可應用於血漿及腦脊髓液中piracetam定量分析研究，並應用於臨床病人之檢體。

關鍵字

微胞電動層析法 ； amikacin ； kanamycin A ； tobramycin ； piracetam

並列摘要

The purpose of this work is directed to develop the accurate and rapid methods for the different medicines by micellar electrokinetic chromatography. The main studies included simultaneous determination of amikacin, kanamycin A and tobramycin, and analysis of piracetam in human plasma and in cerebrospinal fluid (CSF) by micellar electrokinetic chromatography with direct injection. The results are summarized as follow：

1. Simultaneous Determination of Amikacin, Kanamycin A and Tobramycin by Micellar Electrokinetic Chromatography and Application in Drug Formulations (Electrophoresis, 2005, 26, 947-953.)
A simple micellar electrokinetic chromatography is described for simultaneous determination of amikacin, kanamycin A and tobramycin. The analysis was performed in Tris buffer (180 mM; pH 9.1) with 300 mM sodium pentanesulfonate as a background electrolyte. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and concentrations of sodium pentanesulfonate. Application of the method to the determination of amikacin, kanamycin A and tobramycin in commercial formulations was proved to be feasible.

2. Determination of Piracetam in Human Plasma and CSF by Micellar Electrokinetic Chromatography with Direct Injection
A simple micellar electrokinetic chromatography is described for determination of piracetam in human plasma and in CSF. The method was performed in Tris buffer (30 mM; pH 9.5) with 280 mM sodium dodecyl sulfate as a background electrolyte. Several parameters affecting the separation of the drug were studied, including the pH and concentrations of the Tris buffer and concentrations of sodium dodecyl sulfate. Application of the proposed method to the determination of piracetam in patients’ plasma and CSF was proved to be feasible.

並列關鍵字

micellar electrokinetic chromatography ； amikacin ； kanamycin A ； tobramycin ； piracetam

參考文獻

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