女性純粹毒品濫用者、合併使用毒品與檳榔者周邊血液單核細胞分泌細胞激素能力之研究

The ability on the release of cytokines by cultured peripheral blood mononuclear cells from female absolute drug abusers and drugs combined with betel quid use

鄭世佳(Shih-Chia Cheng)

指導教授 ： 謝天渝 ； 張基隆

高雄醫學大學/口腔醫學院/牙醫學研究所/碩士(2005年)

摘要

中文摘要
在台灣的男性毒品濫用者86.97%伴隨有嚼食檳榔的習慣，而台灣的女性毒品濫用者，約有55.8%具嚼食檳榔的習慣，毒品及嚼食檳榔皆會造成調控免疫系統細胞激素的改變，本研究主要探討女性嚼食檳榔與毒品濫用對細胞激素的影響。
本研究分為純粹毒品(安非他命、海洛因)濫用組38例、合併使用毒品與檳榔組10例兩組實驗組，對照組為沒有毒品濫用及嚼食檳榔習慣者16例，取周邊靜脈血分離培養單核細胞72小時後，以酵素免疫法測定單核細胞分泌之細胞激素，結果顯示正常組IFN-γ的分泌濃度大於純粹毒品濫用組又大於合併使用毒品與檳榔組；正常組TGF-β的分泌濃度大於合併使用毒品與檳榔組又大於純粹毒品濫用組，而正常組IFN-γ、TGF-β的分泌濃度，在統計上顯著性大於純粹毒品濫用組(IFN-γ: 142.46±53.79>52.90±8.74, p<0.05;TGF-β:611.90±27.55> 508.21±19.92, p<0.05)。在加入刺激因子PHA後，正常組IFN-γ和TGF-β分泌濃度均在統計上顯著大於純粹毒品濫用組(IFN-γ: 469.84±125.08>157.81±39.82, p<0.05;TGF-β: 589.85±24.01>521.16±15.61, p<0.05)。在比較單核細胞分泌能力(加入刺激因子前之分泌濃度除以加入刺激因子後之分泌濃度)，純粹毒品濫用組、合併使用毒品與檳榔組在TGF-β的分泌能力和正常組無明顯差異，而IFN-γ的分泌能力正常組高於純粹毒品濫用組、合併使用毒品與檳榔組，且正常組在統計上顯著性大於合併使用毒品與檳榔組(7.26±1.88>1.46±0.40, p<0.05)。因此由本研究的實驗結果推論，毒品會影響單核細胞細胞激素IFN-γ、TGF-β的分泌，在IFN-γ方面：毒品會降低單核細胞分泌IFN-γ的濃度，而合併毒品與檳榔使用者其單核細胞分泌IFN-γ的濃度會更低，由於IFN-γ是一種免疫活化因子，顯示合併使用毒品與檳榔者和純粹毒品使用者相比，免疫活化程度較低；在TGF-β方面：毒品也會降低單核細胞分泌TGF-β的濃度，而合併毒品與檳榔使用者其單核細胞分泌TGF-β的濃度會昇高，由於TGF-β是一種免疫抑制因子，顯示合併使用毒品與檳榔使用者會比純粹毒品使用者，更存在某種程度的免疫抑制反應，故本研究推論檳榔對毒品的免疫抑制作用有某種程度的加成效果。

關鍵字

細胞激素 ； 檳榔 ； 毒品濫用

並列摘要

Abstract

Both betel quid chewing and drug abuse change cytokines secretion that modulate the immune system. 86.97% of male drug abusers in Taiwan have the habit of betel quid chewing, however, only 55.8% of female drug abusers in Taiwan have the habit of betel quid chewing. We can take advantage of Taiwanese females special habits of drug abuse and betel quid chewing to investigate the effects of cytokines release from absolute drug abusers and drugs combined with betel quid use.
This study divided the groups into absolute drug(amphetamine, heroin) abusers (38 cases),and drugs combined with betel quid user (10 cases). The control group included 16 cases without drug abuse or betel quid chewing. After culturing the peripheral blood mononuclear cells for 72 hours, we detected the release of cytokines with ELISA. The results revealed that the amount of IFN-γ from the control group was significantly higher than the group of absolute drug abusers and that was higher than drugs combined with betel quid use. The amount of TGF-β from the control group was significantly higher than the group of drugs combined with betel quid use and that was higher than absolute drug abusers. (IFN-γ: 142.46±53.79>52.90±8.74, p<0.05;TGF-β:611.90±27.55> 508.21±19.92, p<0.05) After adding PHA, the amount of IFN-γ from the control group was significantly higher than the group of absolute drug abuser. The amount of TGF-β from the control group was significantly higher than the group of absolute drug abuser.(IFN-γ: 469.84±125.08>157.81±39.82, p<0.05;TGF-β: 589.85±24.01>521.16±15.61, p<0.05) Comparing the secretion ability of mononuclear cells, the effect of TGF-β from absolute drug abusers and drugs combined with betel quid use was not different from the control group. The effect of IFN-γ from absolute drug abusers and drugs combined with betel quid use was lower than the control group and the control group was significantly higher than the group of drugs combined with betel quid use. (7.26±1.88>1.46±0.40, p<0.05) Based on our results, drugs affect the IFN-γ and TGF-β secretion ability of mononuclear cells. As for IFN-γ, drugs can decrease the IFN-γ secretion concentration of mononuclear cells and the IFN-γ secretion concentration of mononuclear cells of drugs combined with betel quid use was lower than absolute drug abusers. However, IFN-γ is an immune activator, the immune activity of drugs combined with betel quid use is lower than absolute drug abusers. In TGF-β, drugs can also decrease the TGF-β secretion concentration of mononuclear cells and the TGF-β secretion concentration of mononuclear cells of drugs combined with betel quid use was higher than absolute drug abusers. Because TGF-β is an immune suppresser, we believe there is greater immune suppression in drugs combined with betel quid use. According to our results, the study concludes betel quid has some immune suppressive augmentation to drug abuse.

並列關鍵字

cytokines ； betel quid use ； drug abusers

參考文獻

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