瓊楠根部化學成分與抗結核活性之研究

摘要瓊楠(Beilschmiedia erythrophloia Hay.)為樟科(Lauraceae)常綠喬木，分布於中國南部、海南島、琉球及台灣全島中低海拔森林中。瓊楠屬植物在台灣僅有兩種，從瓊楠根部的甲醇萃取物發現具有抗結核菌活性，且其化學成分從未被研究過，極具研究價值。針對瓊楠根部甲醇萃取物的抗結核菌活性成分進行研究，將瓊楠根部甲醇萃取物進行分配萃取後，得到乙酸乙酯層、水層及不溶物三個部分，其中乙酸乙酯層顯示具有抑制結核菌的活性，遂進行乙酸乙酯層的化學成分研究，共獲得28個化合物，其中有10個新化合物，1個天然首次分離化合物，17個已知化合物︰一、 endiandric acid化合物9個︰erythrophloins A (1) ~F (6)、beilcyclone A (7)、endiandric acids I (8) and J (9)，皆為新化合物。二、 benzopyran化合物3個︰oligandrol (10)、oligandrol methyl ether (11)、 dehydrooligandrol methyl ether (12)，而化合物 (12) 為新化合物。三、 benzenoid化合物2個︰farnesylol (13)、vanillin (17)，而化合物(13) 為天然首次分離。四、 sesquiterpene化合物2個︰caryophyllene oxide (14)、suberosol B (15)。五、 amide化合物1個︰dioxamin (16)。六、 steroid化合物5個︰β-sitostenone (18)、6β-hydroxystigmast-4-en-3-one (19)、 24(S)-3β-hydroxystigmast-5-en-7-one (20)、β-sitosterol (21)、stigmasterol (22)。七、 triterpene化合物2個︰lupeol (23)、3-O-acetyl-epi-betulinic acid (24)。八、 benzoquinone化合物1個︰α-tocopheryl quinone (25)。九、 fatty acid化合物3個︰methyl oleate (26)、methyl palmitate (27)、methyl linoleate (28)。其中化合物13、15具抗結核活性。

關鍵字

瓊楠；抗結核

並列摘要

Abstract Beilschmiedia erythrophloia Hay. (Lauraceae) is an evergreen tree, distributed in south China, Hainan, Ryukyus, and throughout the Taiwan. There are only two species of Beilschmiedia in Taiwan, the methanolic extract from the root of B. erythrophloia showed antitubercular activity, and its chemical constituents have never been conducted. The aim of this study is the isolation of chemical constituents and antitubercular activity from the methanolic extract of the root, which was partitioned to get EtOAc layer, H2O layer and insoluble part. Examination of active EtOAc layer led to the isolation of 28 compounds︰10 new compounds, 1 firstly isolated from nature compound and 17 known compounds. 1. Nine endiandric acids︰erythrophloin A (1)~F (6), beilcyclone A (7), endiandric acids I (8) and J (9). All of them are new compounds. 2. Three benzopyrans︰oligandrol (10), oligandrol methyl ether (11), dehydrooligandrol methyl ether (12). Compound (12) is a new compound. 3. Two benzenoids︰farnesylol (13), vanillin (17). Compound (13) was firstly isolated from nature. 4. Two sesquiterpenes︰caryophyllene oxide (14), suberosol B (15). 5. One amide︰dioxamin (16). 6. Five steroids︰β-sitostenone (18), 6β-hydroxystigmast-4-en-3-one (19), 24(S)-3β-hydroxystigmast-5-en-7-one (20), β-sitosterol (21), stigmasterol (22). 7. Two triterpenes︰lupeol (23), 3-O-acetyl-epi-betulinic acid (24). 8. One benzoquinone︰α-tocopheryl quinone (25). 9. Three fatty acids︰methyl oleate (26), methyl palmitate (27), methyl linoleate (28). Compound 3 and 15 showed antitubercular activity.