九重吹根部之化學成分及抗結核活性之研究

Chemical Constituents and Antitubercular Activity from the Root of Ficus nervosa

陳俐雯(Li-Wen Chen)

指導教授 ： 陳益昇

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2009年)

摘要

九重吹(Ficus nervosa Heyne ex Roth.)為桑科，長綠喬木，主要分布於
中國南方、印度、馬來西亞、台灣低海拔地區。F. nervosa 之根部甲醇萃
取物，對Mycobacterium tuberculosis H37Rv，具體外有抗結核活性，而其化
學成分及生物活性均未被報告過。
將本植物根部之甲醇萃取物進行分配，得到乙酸乙酯及水層，針對乙
酸乙酯層進行研究，迄今分離得到2個新pyranocoumarin，分別為：
3-hydroxyxanthyletin (1) 及3-methoxyxanthyletin (2)。還有22個已知化合
物，其中4個為coumarin: xanthyletin (3), umbelliferone (4), scopoletin (5),
S-(+)-marmesin (6)，9個flavonoid：carpachromene (7), parvisoflavone B (8), ,
alpinumisoflavone (9) , genistein (10), 2´-hydroxygenistein (11), prunetin (12),
cajanin (13), apigenin (14), (2S)-naringenin (15) ， 5 個steroid: ergosterol
peroxide (16), a mixture of 6??-hydroxystigmast-4-en-3-one (17) and
6??-hydroxystigmast-4,22-dien-3-one (18), a mixture of β-sitosterol (19) and
stigmasterol(20)，3個benzenoids: 4-hydroxybenzaldehyde (21), vanillin (22),
(S)-lasiodiplodin (23)，一個 triterpenoid: oleanolic acid (24),所有化合物之結
構均經各種光譜分析予以確認。
3-hydroxyxanthyletin (1), genistein (10), prunetin (12), (2S)-naringenin
(15)對Mycobacterium tuberculosis H37Rv，呈現體外抑制作用，MICs 值分
別為: 16, 35, 30, ≤ 2.8 μg/ mL。

關鍵字

桑科 ； 根部 ； 九重吹 ； 抗結核

並列摘要

Ficus nervosa Heyne ex Roth. (Moraceae) is an evergreen tree, distributed
in south China, India, Malaysia and at low altitudes throughout Taiwan. As an
extension of our continuing studies on the active constituents of Formosan
plants. The methanolic extracts of the root of this species showed antitubercular
activity against Mycobacterium tuberculosis H37Rv in vitro. The aim of this
study is the isolation of chemical constituents and antitubercular activity.
Bioassay-guided fractionation of the active EtOAc soluble layer led to the
isolation of two new pyranocoumarins, 3-hydroxyxanthyletin (1) and
3-methoxyxanthyletin (2), along with twenty-two known compounds, including
four simple coumarins: xanthyletin (3), umbelliferone (4), scopoletin (5),
S-(+)-marmesin (6), nine flavonoids: carpachromene (7), parvisoflavone B (8),
alpinumisoflavone (9), genistein (10), 2´-hydroxygenistein (11), prunetin (12),
cajanin (13), apigenin (14), (2S)-naringenin (15), five steroids: ergosterol
peroxide (16), a mixture of 6??-hydroxystigmast-4-en-3-one (17) and
6??-hydroxystigmast-4,22-dien-3-one (18), a mixture of β-sitosterol (19) and
stigmasterol(20), two benzenoids: 4-hydroxybenzaldehyde (21), vanillin (22),
(S)-lasiodiplodin (23), one triterpenoid: oleanolic acid (24).The structures of
these compounds were determined by spectroscopic analyses.
Among these compounds, 3-hydroxyxanthyletin (1), genistein (10),
prunetin (12), (2S)-naringenin (15), showed antitubercular activity against
Mycobacterium tuberculosis H37RV in vitro with MIC values: 16, 35, 30, ≤
2.8μg/ mL, respectively.

並列關鍵字

Moraceae ； Root ； Ficus nervosa ； Antitubercular activity

參考文獻

1. (a)Lu SY, Yang YP. Moraceae in Flora of Taiwan. 2nd ed. Editorial

Committee of the Flora of Taiwan: Taipei, 1996; Vol.Ⅱ, pp.145-88.

(b)楊遠波，劉和義，呂勝由，台灣維管束植物簡誌，第二卷，行政院

農委會，台北，1997，pp.46-55

2. http://www.doh.gov.tw/CHT2006/DM/SEARCH_RESULT.aspx