南投月桃萃取物對於抗癌與抗發炎的活性探討

Exploring anti-inflammatory and anti-cancer activities for different parts of Alpinia Nantoensis

童大瑋(Ta-Wei Tung)

指導教授 ： 王惠君

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2013年)

摘要

藥物開發中，天然成分代謝產物的多樣性一直是最重要的來源。在自然界植物中，薑科植物應用廣泛於癌症預防和癌症治療其抽提成分中也具強烈抗發炎、抗氧化、抗血小板凝集和抗癌等功效。南投月桃(Alpinia nantoensis)為薑科月桃屬植物，是台灣原生種，分布於台灣中部山區。迄今為止仍未有針對南投月桃的生物活性評估研究被報導，因此本研究主要探討南投月桃之抗癌活性與抗發炎特性。內容主要分成三部分：(一) 南投月桃之抗癌活性及其活性成分、(二) 南投月桃透過抑制癌症細胞轉移能力及其機轉，與(三) 南投月桃及其活性成分抑制LPS誘導RAW 264.7巨噬細胞發炎反應之影響。
　　研究中將南投月桃各個部位萃取物，根莖、莖和葉分別處理人類癌細胞探討其對細胞抑制生長之機制，從實驗結果可以看到南投月桃顯示出低濃度即有很強的效用，具有開發成抗發炎和對抗惡性腫瘤相關疾病的有效活性藥物。

關鍵字

南投月桃 ； 乳癌 ； 轉移

並列摘要

Natural products containing a diversity of metabolites have been the most important source of leads for the development of drugs. The application of extracts from plants of the genus Alpinia plants also have been implicated in chemoprevention and chemotherapy given that their extracts exhibited strong anti-oxidant and anti-cancer activities. Alpinia nantoensis is a folk plant endemic to Taiwan but the information on bioactivities for its metabolites is limited so far.
In this study, we assessed the effects of Alpinia nantoensis extracts on LPS-stimulated NO production in RAW 264.7 macrophages, and determined cytotoxicity and the cell migration and invasion of MDA-MB-231 cancer cell. In MDA-MB-231 cells, the half-maximal effective concentrations of Alpinia nantoensis extracts for cytotoxicity ranged from 28.13 to 58.04 μg/mL. The expression of phospho-AKT was markedly decreased in MDA-MB-231 cells treated with 3 crude extracts might be one of molecular mechanism underlying the anti-cancer activties. Our results showed the extract of A. nantoensis has low toxicity with high effectiveness and development potential to treating inflammatory or malignant diseases.

並列關鍵字

Alpinia nantoensis ； anti-inflammatory ； anti-cancer

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