補骨脂化合物－榛仁球蛋白及新補骨脂異黃酮對於脂多醣誘導發炎反應和巨噬細胞分化的影響

The effects of Psoralea corylifloia L. derived-compounds-corylin and neobavaisoflavone on LPS-induced inflammatory response and macrophage polarization

賴怡欣(Yi-Xin Lai)

指導教授 ： 李佳陽

高雄醫學大學/醫學院/醫學研究所碩士班/碩士(2019年)

摘要

補骨脂是一種重要的中醫藥植物，已使用數千年。其廣泛應用在治療白斑病、皮膚疾病、骨質疏鬆症、心血管疾病及癌症。從補骨脂中分離出的化合物，如補骨脂異黃酮、異補骨脂查爾酮、新補骨脂異黃酮、榛仁球蛋白，都已被證實具有多種藥理活性，包括抗氧化、抗微生物和抗發炎。巨噬細胞為免疫反應的第一道防線，負責吞噬外來病原體，並清除受損及死亡細胞。除此之外，巨噬細胞接收外來刺激，會分化成促發炎M1型態或是抗發炎M2型態。
本篇論文利用脂多醣誘發小鼠巨噬細胞與人類單核球細胞做為發炎細胞模式，藉以探討榛仁球蛋白與新補骨脂異黃酮的抗發炎效果，並分析榛仁球蛋白與新補骨脂異黃酮對於巨噬細胞分化的影響。
實驗結果顯示榛仁球蛋白抑制小鼠與人類巨噬細胞釋放促發炎因子及NLRP3發炎小體活化；新補骨脂異黃酮則是抑制小鼠巨噬細胞釋放促發炎因子及NLRP3發炎小體活化。接下來，我們將進一步找出榛仁球蛋白與新補骨脂異黃酮對於NLRP3發炎小體與巨噬細胞分化影響的作用機制。

關鍵字

巨噬細胞 ； 發炎反應 ； NLRP3發炎小體

並列摘要

Psoralea corylifloia L., an important Chinese herbal plant with thousands of years, has been widely used in the treatment of many diseases including leucoderma, skin diseases, osteoporosis, cardiovascular diseases and cancer. Several compounds isolated from Psoralea corylifloia L., such as bavachin, isobavachalcone, neobavaisoflavone and corylin, have been demonstrated to possess multiple pharmacological activities including anti-oxidantion, anti-microbial action and anti-inflammation. Macrophages, the first line of inflammation response, are responsible for eliminating pathogens and removing damaged or death cells. In addition, response to extrinsic stimuli, macrophages will derive into M1 type (pro-inflammatory) or M2 type (anti-inflammatory).
In the present study, we used LPS-triggered murine macrophages (J774A.1 cells) and human monocytes (THP-1 cells) as inflammatory cell models to examine the anti-inflammatory effects of corylin and neobavaisoflavone. Furthermore, the effects of corylin and neobavaisoflavone on macrophage polarization have been also analyzed.
Experimental results indicated that corylin inhibits pro-inflammatory cytokines secretion and the activation of NLRP3 inflammasome in both murine and human macrophages. Neobavaisoflavone also inhibits pro-inflammatory cytokines secretion and NLRP3 inflammasome activation in murine macrophages. In the future, we will further explore the mechanism of action that corylin and neobavaisoflavone affects the activation of NLRP3 inflammasome and macrophage polarization.

並列關鍵字

Macrophage ； Inflammation ； NLRP3 inflammasome

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