台灣產印度金鈕扣活性化學成分分離及其品質管制之研究

印度金鈕扣(Acmella oleracea)，菊科(Asteraceae)，為一年生草本植物，分布在非洲及南美洲，花為黃色，頭狀花序，民間用於治療牙痛、喉嚨痛、口腔炎…等。烷基醯胺類化合物(alkylamides)為A. oleracea之主要成分，被報導出有利尿、抗菌、抗發炎的效果。此外，此類化合物，由於有抗皺紋的效果，被廣泛添加在皮膚保養品，但缺乏此類化合物之品質控制之平台。本研究中，首先建立了一個快速獲得富含烷基醯胺類化合物(alkylamides)之濃縮層方法。此外，將台灣產此植物花部以乙醇萃取，利用活性導引法進行活性成分分離，成功分離出7個化合物，一組steroid 混合物: β -sitosterol β-D-glucoside (1)與stigmasterol-β-D-glucoside (2)，其中包含5個烷基醯胺類化合物: spilanthol金鈕扣醇 (3)、(2E,6Z,8E)-N-(2-methylbutyl)-2,6,8-decatrienamide (4)、(2E,5Z)-N-isobutyl-2,5-undecadiene-8,10-diynamide (5)、(2E)-N-isobutyl-2-undecene-8,10-diynamide (6)、(2E)-N-(2-methylbutyl)-2-undecene-8,10-diynamide (7) ，其中化合物5為新化合物。本研究同時利用HPLC-PDA 檢測相關成份，並開發了一種快速簡便方法，在十五分鐘內針對本植物花部、地下部、地上部及同屬不同種之植物天文草(Acmella ciliata)花部及地上部成分之定性定量分析，發現化合物3在印度金鈕扣花部具有最高含量而，化合物4在其地上部及地下部具有最高的含量；此外天文草中的spilanthol (3)比印度金鈕扣含量較少，且活性成分大多位於花部。最後，進一步探討印度金鈕扣成分之安定性，此研究提供了一個有效快速方法訂定出具代表性的化學圖譜、檢測印度金鈕扣活性成分烷基醯胺類化合物之品質控制平台，更可應用於印度金鈕扣相關產品。

關鍵字

金鈕扣；印度金鈕扣

並列摘要

Acmella oleracea (AO) is an annual herb native to the tropical parts of Africa and South America with a yellow flower head, which is commonly used in traditional folk medicine to cure toothaches, throat complaints, and stomatitis. Alkylamides, one category of AO major active components, are regarded for their diuretic, antibacterial, and anti-inflammatory activities. Besides the medicinal use, alkylamides are well-known for their anti-wrinkle activity and used in different skin care products. Despite the extensive use in cosmetic industry, the production and marketing of AO and its alkylamides face a critical threat due to the lack of a reliable quality assessment method to evaluate concentration of alkylamides. In this investigation, we report a convenient method for concentrating the alkylamide-rich fraction (ARF), from the ethanolic extract of AO flowers (EEF-AO). Using bio-activity guided isolation technique, five alkylamides, including spilanthol (3), (2E,6Z,8E)-N-(2-methylbutyl)-2,6,8-decatrienamide (4), (2E,5Z)-N-isobutylundeca-2,5-diene-8,10-diynamide (5), (2E)-N-isobutyl-2-undecene-8,10-diynamide (6), (2E)-N-(2-methylbutyl)-2-undecene-8,10-diynamide (7), and a mixture of steroids 1-2, compound 5 is new. To detect the concentration of these markers in different plant parts, a rapid HPLC-PDA method for simultaneous detection of alkylamides from AO and A. ciliata (AC) was established. Two major alkylamides 3 - 4 were separated as the chemical markers of ARF. Using the developed HPLC-PDA protocol, the contents differences of major alkylamides in different part of AO and AC were achieved. Utilizing the efficient extraction, concentration and rapid analysis method not only provide necessary information about AO alkylamides concents in different plants parts but also provide a quality control assessment of AO alkylamides during different harvesting times.

並列關鍵字

Acmella ； Acmella oleracea ； alkylamides

參考文獻

第九章參考文獻

Google Scholar

1. Khan IA, Smillie T. Implementing. a "quality by design" approach to assure the safety and integrity of botanical dietary supplements. J Nat Prod., 2012, 28:1665-1673.