大黃、黃水茄、香樁、蛇莓抗非荷爾蒙敏感性前列腺癌及機制探討

The antitumor activity of Emodin, Solamargine, Toona Sinensis, Duchsnea Chrysantha on nonhormone sensitive prostate cancer cell lines

方家賢(Chia-Hsiang Fang)

指導教授 ： 蔡錦蓮

高雄醫學大學/生命科學院/醫藥暨應用化學研究所碩士在職專班/碩士(2007年)

https://doi.org/10.6832/KMU.2007.00146

摘要

抗癌藥物所引起細胞凋亡機制，是眾多學者努力研究的方向。許多的抗癌藥物，都具備誘發腫瘤細胞-自然凋亡的作用。如：大黃萃取液對於男性前列腺癌及人類肺腺癌癌細胞有極佳的抑制效果。黃水茄萃取液對於人類肝癌及肺癌細胞有極佳的抑制效果。香樁萃取液對於女性卵巢癌細胞、男性睪丸間質細胞癌及人類肺癌細胞的增生，有極佳的抑制效果。蛇莓萃取液對於白血病細胞及人類癌細胞有極佳的抑制效果。目前美國男性十大癌症中，不論是前列腺癌的發生率或是死亡率，都高居第一位。東方的黃種人罹患前列腺癌的比率不高，但是這幾年來，國內前列腺癌死亡率，今年上升為第七名，且有逐年增加的趨勢。

本研究是利用非依賴型-荷爾蒙PC-3、DU-145癌細胞株為研究題材，來探討大黃(Emodin)、黃水茄(Solamargine)、香椿(Toona Sinensis)，以及蛇莓(Duchsnea Chrysantha)等中草藥，對於抑制-前列腺癌的毒殺效果與機制。由MTT實驗結果顯示，大黃對PC-3在50%的存活率的濃度為228 ppm。對DU-145在50%的存活率濃度為223 ppm。黃水茄對PC-3在50%的存活率濃度為35 ppm。對DU-145在50%的存活率濃度為28 ppm。香椿對PC-3在50%的存活率濃度為400 ppm。對DU-145在50%的存活率濃度為302 ppm。蛇莓對PC-3在50%的存活率濃度為327 ppm。對於DU-145在50%的存活率濃度為193 ppm。結果顯示黃水茄對PC-3的毒殺效果最好，香椿為次之，毒殺效果最差的為蛇莓，結果顯示黃水茄對DU145的毒殺效果最好，香椿為次之，毒殺效果最差的是蛇莓，且黃水茄對於PC-3與DU-145的比較之下，在DU-145有很好的抑制效果。由此可知 DU-145對四種藥物的敏感性大於PC-3。

細胞螢光免疫實驗結果顯示PC-3與 DU-145在大黃、黃水茄、香椿、蛇莓作用有P21蛋白質有極強的表現；在PCNA及MDM2在上述四種中草藥的蛋白質螢光表現上較P21差；在TRADD、FADD、BCL2、BAX、Caspase 3、Caspase 8、Caspase 9等細胞凋亡有關的蛋白在四種中草藥的無表現。由此結果可推測大黃、黃水茄、香椿、蛇莓藉由P21的蛋白誘發引起細胞核停止分裂，而造成癌細胞數目不再增加。

關鍵字

大黃 ； 黃水茄 ； 香樁 ； 蛇莓 ； 前列腺癌細胞株

並列摘要

Mechanism that dies that the cell that the anticancer drug causes withers， it is the direction that numerous scholars make great efforts to study。 Though a lot of anticancer drug， possess and bring out the tumour cell - the function that withers and dies naturally 。 If fragrant of leaf water assemble thing (TSL-CE ) to human lung hyperplasia ， cancer cell of A549 ， there are apparent restrain results； A549 is a type in the non- little cell lung cancer (NSCLC )， the non- little cell lung cancer (NSCLC ) includes: Human lung gland cancer cell (H441 ) lung scale's form cancer cell (H520 ) and big cell's cancer cell of lung (H661 )。 Composition of TSL-1 among them of the thing (TSL-CE ) that the fragrant leaves water assembleses， have extremely good restraining the cancer result and bringing out cells to wither and die to the big cell's cancer cell of lung (H661 ) even more。

It has cancer of resisting that the rhubarb (Emodin ) is verified， physiological activation ， such as antivirus ，etc。； Discover in the near future ， Emodin can also suppress the activation of breast cancer cell Tyrosine Kinase ， and have a very strong restrain result to T - the lymphocyte。 Moreover， the rhubarb is plain and can be brought out the withering to die naturally of human lung cancer cell by the report too； Direct against the human liver tumour cell: HepG2/C3A， the rhubarb of
result of killing of PLC/PRF/5 and SK-HEP-1 is plain to represent extremely good function， especially bring out on HepG2/C3A cell。

The scientist finds from take a photograph of the hereditary family which protects the gland cancer， the defect of known gene RNASEL， infer that the defect of this gene results in taking a photograph of and protecting the gland cancer， should have something to do with the function that He Er is covered with 。 Though in the position of the first pair of chromosome ， find out a section of defects position ， and is named HPC1 (hereditary prostate cancer 1 )， but has not found that there is any gene that relates to take a photograph of and protect the gland cancer。

In the lung cancer cell with fragrant one- rhubarb- the mock-strawberry， the tumour of the liver cancer cell is suppressing the apparent result of killing， and then protect the gland cancer in taking the photograph and carry on relevant experiment and discussion.

並列關鍵字

Emodin ； Solamargine ； Toona Sinensis ； Duchsnea Chrysantha ； Prostate cancer cell lines

參考文獻

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