台灣產丹參指標成分分析之研究

Quality Control by using HPLC-PDA-approached for the determination biomarkers from Salvia miltiorrhiza (Tanshen) products in Taiwan

徐福蘭(Fu-Lan Shu)

指導教授 ： 曾誠齊

共同指導教授 ： 張芳榮(Fang-Rong Chang)

高雄醫學大學/生命科學院/醫藥暨應用化學研究所/碩士(2010年)

摘要

摘要
丹參，為唇形科(Lamiaceae)植物(Salvia miltiorrhiza)之根莖部，原產於中國，為一種常用的中藥。由於丹參具有顯著及明確的生物活性，特別適用於治療心血管疾病，因此，這幾年西方國家已廣泛接受並作為健康食品。研究中指出，丹參藥材中主要含有兩類的活性成分，分別是親酯性的丹參酮及親水性的丹參酚酸衍生物。其中，丹參酮IIA及丹參酚酸B各為抗癌及抗氧化的活性指標成分。
在台灣，除了作為藥用外，丹參也因其香味而著名，常用於藥膳料理。近年來，由於中國政府限制其總量出口，讓丹參身價水漲船高。此外，由中國進口之藥材，尚存農藥、重金屬殘留，以及中藥材混用等食品安全的問題，仍然讓台灣消費者憂心。有鑑於此，農委會花蓮農業改良場為建立台灣本土化的中草藥產業，依適合台灣之栽培環境，選定丹參作為高產值農作物，經過六年的研發，在花蓮地區成功地大規模種植。
於本次研究中，完成了不同來源丹參產品之定性及定量分析。丹參樣品由台灣生物科技公司(A)所提供，另於高雄購買市售之中國丹參乾品。我們使用高效能液相層析儀結合光電二極體陣列偵測器(Photodiode-array spectrometer, PDA) ，作為偵測兩個活性指標成分的依據。由分析結果得到以下結論:就指紋圖譜而言，台灣丹參鮮品與中國丹參之產品非常相似，就兩個活性指標成份含量來看，台灣的丹參鮮品頗具競爭性。

關鍵字

丹參 ； 丹參酮IIA及丹參酚酸B ； 定性及定量分析

並列摘要

Abstract
Tanshen, the rhizome of Salvia miltiorrhiza, is one of the most commonly used traditional Chinese Medicines. It is widely accepted as a health product in the western countries in recent years owing to its remarkable and reliable biological activities, especially in the treatment of cardiovascular disorders. There are two classes of major active constituents reported in Tanshen, lipophilic tanshiones and hydrophilic polyphenols. Among them, tanshione IIA and salvianolic acid B are the two major bioactive markers highly regarded for their anti-cancer and anti-oxidant activities, individually.
Besides the medicinal use, Tanshen is well-known for its aroma used as a source of perfume ingredients in Taiwan. However, the price of Tanshen is rapidly increasing due to the limitations in import from China, the main producer of S. miltiorrhiza rhizomes. The problems of food safety, including the residues of pesticide, heavy metals, and also the misuse of the original herbal medicines, were still concerned for Taiwanese consumers. In establishment of the industry of traditional herbal medicine, cultivated S. miltiorrhiza in Taiwan was selected and developed as the treasure crop by Hualien Prefectural Agricultural Experiment Station (HPAES) on the basis of adaptation to local conditions. Nowadays, the extensive cultivation of Tanshen is successfully taking place in Hualien based after six years of research and development. Tanshen products are used in cooking as fresh food material, and can be used for traditional Chinese medicine and biotechnological products.
In this study, qualitative and quantitative analysis of various Tanshen products from different sources were obtained. The identification of several bioactive markers (tanshione IIA and salvianolic acid B) was evaluated and comparied by high-performance liquid chromatography with a photodiode-array detector (HPLC-PDA). The results showed that the HPLC fingerprints of Tanshen products from different sources were investigated, and the content of tanshione IIA and salvianolic acid B in Taiwanese S. miltiorrhiza was competitive to those from different origins.

並列關鍵字

Tanshen ； Salvia miltiorrhiza ； tanshione IIA and salvianolic acid B ； qualitative and quantitative analysis

參考文獻

第七章、參考文獻

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