探討HZY-2023在攝護腺細胞系的毒殺作用

The cytotoxicity of HZY-2023 in prostate cancer

劉柏君(Po-Cuhn Liu)

指導教授 ： 王記慧

高雄醫學大學/醫學院/生物化學研究所/碩士(2006年)

摘要

中文摘要
我們從200多種有機合成的化合物，篩選出HZY-2023
2-((E)-2-(5-nitrofuran-2-yl)vunyl)quinolin-8-ol。再藉由ATP asaay分析的方式，篩選出化合物並且找出它的IC50=0.02uM。而細胞凋亡早期特徵早期特徵為細胞膜內側的磷脂(phosphtidylserine ,PS)會外翻至細胞膜外側，晚期特徵為PARP (poly(ADP-ribose)polymerase)的裂解。我們經由流式細胞儀和西方點墨方偵測出這兩種細胞凋亡的特徵。證實HZY-2023會導致PC-3攝護腺癌的細胞凋亡。之後，在再以流式細胞儀，觀察細胞週期的變化。經過上面的實驗得知，HZY-2023能毒殺PC-3攝護腺癌細胞，引發細胞週期停留在G1期，並促使細胞凋亡的發生。

關鍵字

細胞凋亡 ； 細胞週期

並列摘要

Abstract
The compound 2-((E)-2-(5-nitrofuran-2-yl)vunyl)quinolin-8-ol, HZY-2023, was screened from two hundred synthesized chemicals by cytotosic activity assay. The IC50 value of HZY-2023 is about 0.2uM for androgen – independent prostate cancer cell lines, PC3. Using annexin-V staining and the cleavage pattern of poly(ADP-ribose)polymerase indicated that HZY-2023 induce PC3 cell to go into apoptosis. To further delineate the mechanism of apoptosis induced by this compound, we perfomed cell cycle analysis with flow cytometry. The results demonstrated that HZY-2023 causes PC3 cell division to arrest at G1 phase. Together, our finding suggested, the HZY-2023 can, provoke the cell cycle arrest at G1 phase in PC3 cell, resulting in cell death through apoptosis.

並列關鍵字

apoptosis ； cell cycle

參考文獻

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