建立尿液中多環芳香烴代謝物分析方法用以評估一般人多環芳香烴暴露之研究

Establish of the urinary PAH metabolite analysis method for assessing general population PAH exposure

吳芸臻(Yun-Chen Wu)

指導教授 ： 彭瓊瑜

高雄醫學大學/健康科學院/職業安全衛生研究所/碩士(2009年)

摘要

多環芳香碳烴化合物(PAHs)廣泛分布於空氣、食物、土壤等，PAHs是由有機物燃燒不完全所產生，PAHs污染來源如：汽機車、工廠排放廢氣及木材燃燒、吸煙與廚房油煙等。
本研究建立尿中PAHs 代謝物之分析方法，運用少量的尿液(1.5 mL)與高效率液相層析儀/螢光偵測器，以一次分析四種1-naphthol、2-naphthol、9-phenanthrol和1-hydroxypyrene 的PAHs 代謝物。評估結果包括：檢量線線性迴歸係數R＞0.995。偵測下限1-naphthol為348 ng/L，2-naphthol為230 ng/L，9-phenanthrol為71 ng/L與1-hydroxypyrene為46 ng/L；回收效率82.97 % ~ 107.85 %。
將此方法應用於實際尿液PAHs 代謝物之量測，取1.5 mL尿液，經過前處理、淨化與濃縮等前處理步驟。分析的四種代謝物中，1-naphthol檢出比率為67.5 %，1-hydroxypyrene檢出比率為90 %，然而2-naphthol 與9-phenanthrol檢出率不佳，需於前處理及儀器分析過程中稍作改善，增加此兩種物質檢測出來的機會。

關鍵字

多環芳香烴代謝物 ； 生物偵測 ； 高效率液相層析儀/螢光偵測器

並列摘要

Polycyclic aromatic hydrocarbons (PAHs) are a group of chemicals with two or more than two aromatic rings and widely distributed in air, in food, in soil, and in many occupational environments. PAHs form during incomplete combustion, such as smoking, cooking oil fumes. Some PAHs are considered as carcinogens or suspected carcinogens, such as benzo[a]pyrene and chrysene. Due to their ubiquitous presence and health effects, PAHs draw the public’s attention. Many studies have investigated the external and internal PAH exposure in occupational environments. Few studies examine the PAH exposure of the general population. Therefore, this study tries to establish a method to estimate internal PAH exposures of the general population.

This study establishes the urinary PAH metabolite analysis method for assessing general population PAH. Four hydroxyl-PAH, which are 1-naphthol, 2-naphthol, 9-phenanthrol and 1-hydroxypyrene representing naphthalene, phenanthrene, and pyrene metabolites, respectively, are analyzed by High Performance Liquid Chromatograph/Fluorescence Detector. The linearity (expressing as R), limit of detection (LOD) and reproducibility (expressing as COV) are 0.996, 46 ~ 348 ng/L and 83.0 % ~ 107.9 %, respectively.

Spot urine was collected from the test subject. Before analysis, 1.5-mL thawed urine was pretreated, purified, and condensed. The condensed extracts were quantitatively determined using the established method. The detection percentages of 1-naphthol, 2-naphthol, 9-phenanthrol, and 1-hydroxypyrene are 67.5 %, 5 %, 5 %, and 90 %, respectively. This method is good for detection of 1-naphthol and 1-hydroxypyrene. However, detection of 2-naphthol and 9-phenanthrol is fair, more advanced analysis techniques, such as high resolution gas chromatogram/mass spectrometry, are suggested to improve detection percentages of 2-naphthol and 9-phenanthrol.

並列關鍵字

Polycyclic aromatic hydrocarbons metabolite ； Biological monitoring ； HPLC/FLD

參考文獻

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