應用於厭氧環境下細菌培養之無線傳輸型生物反應器

Wireless bioreactor for anaerobic cultivation of bacteria

林鼎舜(Ding-Shun Lin)

指導教授 ： 楊雅棠

國立清華大學/電機資訊學院/電子工程研究所/碩士(2019年)

摘要

厭氧型細菌在微生物學裡是一門擁有廣泛興趣的研究，然而其無氧培養技術仍然十分地不便利。此論文裡我們呈現了一項技術可以進行厭氧培養的生物反應器，濁度測量、流體攪拌、氣體傳送以及資料無線傳輸等功能均在封閉的厭氧環境完成。其無氧環境是藉由氣體產氣包而非常見生物實驗室裡的厭氧操作台來達成。此研究以大腸桿菌進行培養並藉由不同碳源和氫氣當作能量來源來呈現。

關鍵字

厭氧型細菌 ； 生物反應器 ； 資料無線傳輸 ； 大腸桿菌 ； 氫氣

並列摘要

Anaerobic bacteria are of great interests in wide range of microbiology and yet anaerobic cultivation technique remains cumbersome. Here we report an alternative cultivation strategy based on a bioreactor with the entire operation including turbidity measurement, fluid mixing, gas delivery and data transmission in a gas tight anaerobic enclosure. The anaerobic condition is achieved with gas pack with no need of anaerobic cabinet. Such a cultivation strategy is demonstrated with Escherichia coli with different carbon sources and hydrogen as energy source.

並列關鍵字

anaerobic bacteria ； bioreactor ； data transmission ； Escherichia coli ； hydrogen

參考文獻

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