批次填充與平行蓋印蛋白質溶液之微陣列壓印晶片系統

本論文研發新式之微陣列壓印晶片系統, 能一次同時填充數十種蛋白質溶液, 並將此數十種檢體同時平行蓋印出來. 藉由微陣列填充晶片與微陣列壓印晶片之結合, 數十種蛋白質溶液以毛細力平行傳入到微壓印晶片上方; 而各檢體溶液間, 彼此區隔良好. 各檢體溶液流到填入其相對應的導棒及微流道內, 並流入微壓印章. 將微陣列壓印晶片與底材接觸, 數十種蛋白質溶液平行同時蓋印出來形成蛋白質微陣列. 改善背後填充式微陣列壓印晶片之Su8製程, 作出通的背後填充式微陣列壓印晶片. 可同時蓋印出四種檢體, 蓋印出的液珠尺寸為50μm, 且蓋印尺寸差異性小於10%, 均勻性良好. 此外, 並進一步探討蓋印在不同親水程度表面上, 以及不同黏滯性的檢體溶液對蓋印尺寸結果的影響. 實驗結果顯示蓋印在越親水的表面時, 液珠尺寸越大. 而且當所用的檢體溶液黏滯性較大時, 蓋出的液珠尺寸較小. 所得數據可供壓印晶片設計及蓋印蛋白質微陣列點能有最小間距最大密度之設計參考. 再者, 設計新式的導棒式微陣列壓印晶片, 可與微陣列填充晶片成功整合, 能平行填充及蓋印36種檢體. 利用微轉印法或微流道法製作Teflon 疏水區隔層於晶片表面, 能有效區隔各檢體溶液在填充時, 使其各自填入相對應的導棒, 不與鄰近的檢體混流, 使微陣列填充晶片與微壓印晶片能順利整合在一起, 達到快速批次填充的目的. 並用PDMS微壓印端直接與微填充晶片接合, 沾取檢體後蓋印. 可同時蓋出36種不同的蛋白質檢體溶液, 改善傳統PDMS壓印一次只能轉印一種或幾種的缺點, 且製程容易. 並且研發出新式排針式微陣列壓印晶片, 檢體一次吸取填充後, 可連續蓋印一百次. 蓋印出來各點尺寸及螢光強度均勻性良好, 差異性均小於10%, 且晶片製作簡單.

關鍵字

微陣列；微壓印；蛋白質微陣列

並列摘要

This work develops a novel micro contact printing system for printing tens of protein solutions into an array with batch filling and parallel printing. This printing system consists of a micro filling chip and a micro stamp chip. The micro filling chip can simultaneously transfer numerous protein solutions into the micro stamp chip in seconds by capillary force without cross-contamination, while preserving the functionality of proteins. Different proteins can be dispensed into the corresponding channels and driven into the tips of the micro stamps. The micro stamp can be then brought to contact with the substrate to produce bio-fluid spot arrays. Teflon patterns are applied on both the micro filling chip and the micro stamp chip to prevent cross-contamination during filling. Thirty-six proteins can be printed in parallel with a spot size variation of less than 5%. This device has a potential to be expanded to a passive and high throughput system for simultaneously printing hundreds of bio-fluid spots to form dense arrays for diagnosing disease or screening for drugs.

並列關鍵字

Micro Array ； Micro contact printing ； Protein microarray

參考文獻

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[4] Yoonsuk Lee, Eun Kyoung Lee, Yong Wan Cho, Takuya Matsui, In-Cheol Kang, Tai-Sun Kim, Moon Hi Han, “ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein immobilization for application of protein-protein interaction studies”; Proteomics, 2003, v3, p2289-2304

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批次填充與平行蓋印蛋白質溶液之微陣列壓印晶片系統

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