開發三向功能的奈米探針, 使 SERS (表面增強拉曼光譜) 於無背景干擾下偵測細胞表面的唾液酸

Development of a Tri-Functional Nanoprobe for Background-Free SERS (Surface-enhanced Raman Spectroscopy) Detection of Sialic Acid on Cell Surface

芮娜妲(Renata, Septila)

指導教授 ： 林俊宏 ； 潘榮隆

國立清華大學/生命科學院/生物資訊與結構生物研究所/博士(2021年)

摘要

細胞表面的唾液酸在許多生理和病理學過程中都是不可缺少的，唾液酸的靈敏且可重複的檢測對於許多疾病的診斷和治療至關重要。在本論文中，我們開發了一種三重功能的奈米探針，作為靈敏、簡單的表面增強拉曼光譜（SERS）奈米探針，用於細胞表面上的唾液酸檢測。此報導分子的設計旨在提供三個關鍵功能，使其成為唾液酸檢測的理想選擇。首先，我們採用了兩個識別基團，即苯基硼酸和一個銨基，它們可增強唾液酸的識別和捕獲效率。其次，使用氰基作為拉曼光譜報告分子，因為其分子振動光譜會落在細胞拉曼無聲區中。最後，硫醇充當錨定劑以使報告分子能與銀奈米立方體結合，從而達成SERS並保護銀奈米立方體免於氧化。我們的分子奈米探針設計證明了能夠以高靈敏度和選擇性檢測細胞表面的唾液酸，為細胞診斷開闢了新途徑。

關鍵字

表面增強拉曼光譜

並列摘要

Sialic acids, especially those on cell surface, are indispensable in numerous physiological and pathological processes. Sensitive and reproducible detection of sialic acid is crucial for diagnosis and therapy in many diseases. In this thesis, we developed a tri-functional nanoprobe as a sensitive and straightforward surface-enhanced Raman spectroscopy (SERS) nanoprobe for detecting sialic acid-containing glycan on cell surfaces. The reporter was designed to provide three key functionalities that make it ideal for sialic acid detection. First, we employed two recognition groups, phenylboronic acid and an ammonium group, to enhance sialic acid recognition and capture efficiency. Second, we used cyano as the Raman reporter because its vibrational energy is shown in the cellular Raman silent region. Finally, thiol acted as an anchoring agent that facilitate the conjugation of reporter onto silver nanocubes to provide SERS enhancement and protect silver nanocubes from oxidation. Our molecular nanoprobe design demonstrated the ability to detect sialic acid on the cell surface with high sensitivity and selectivity, opening up new routes to cellular diagnostics.

並列關鍵字

Surface-enhanced Raman Spectroscopy ； Boronic Acid ； Sialic Acid ； Cyano

參考文獻

References

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3. Zhou, X.; Yang, G.; Guan, F., Biological functions and analytical strategies of sialic acids in tumor. Cells 2020, 9, 273.

4. Smith, E.; Dent, G., Introduction, basic theory and principles. In Modern Raman spectroscopy – a practical approach, John Wiley and Sons Ltd: West Sussex, England, 2005, 1-21.